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中国农学通报 ›› 2015, Vol. 31 ›› Issue (11): 118-123.doi: 10.11924/j.issn.1000-6850.casb14120153

所属专题: 生物技术

• 生物技术科学 • 上一篇    下一篇

完全水淹对黄菖蒲幼苗生长和生理的影响

秦洪文,刘正学,郑丽丹,钟 彦,刘 锐,余 建   

  1. 重庆三峡学院生命科学与工程学院,重庆三峡学院生命科学与工程学院,重庆三峡学院生命科学与工程学院,重庆三峡学院生命科学与工程学院,重庆三峡学院生命科学与工程学院,重庆三峡学院生命科学与工程学院
  • 收稿日期:2014-12-23 修回日期:2015-02-13 接受日期:2015-02-25 出版日期:2015-05-06 发布日期:2015-05-06
  • 通讯作者: 刘正学
  • 基金资助:
    国家自然科学基金面上项目“三峡库区消落带种子库动态及多年生草本植物适应机制”(31270451);重庆市教委科学技术研究项目“三峡水库消落带经济植物枸杞适应机制研究”(KJ131103);三峡库区水环境演变与污染防治重点实验室开放基金“三峡水库平坝缓坡消落带湿地构建关键技术研究与示范”(WETKL2012MS-07)

Influence of Submergence on Growth and Physiology of Iris pseudacorus Seedlings

  • Received:2014-12-23 Revised:2015-02-13 Accepted:2015-02-25 Online:2015-05-06 Published:2015-05-06

摘要: 旨在筛选三峡库区消落带适生植物,测定完全水淹对黄菖蒲幼苗生长和生理的影响。在实验室条件下将黄菖蒲幼苗完全水淹0、10、20、30、40天,分析植株形态特征和生理特性变化。结果表明水淹40天,植株株高、叶片数、叶面积、根数和根体积分别为11.53cm、4.00片/株、9.94cm2、5.33根/株和0.18cm3,显著低于对照植株;叶绿素a、叶绿素b和叶绿素a+b含量显著下降,分别为0.26mg/g、0.10mg/g和0.35mg/g;叶绿素a/b、MDA和可溶性糖含量显著上升,分别为2.76、0.0245μmol/L和95.11mg/g;淀粉含量在处理20天上升至最大值37.67mg/g后下降,处理40天降至34.56mg/g。完全水淹显著抑制植株形态生长,使叶绿素被降解,膜脂过氧化程度加深,但植株通过提高叶绿素a/b以及直接供能物质含量,以适应水下能量需求。

关键词: 藏香原料, 藏香原料, 青霉菌, 曲霉菌, 抑菌效果

Abstract: To select adaptable plant to grow in water- level- fluctuating zone of Three Gorges reservoir, the influence of submergence on growth and physiology of Iris pseudacorus was investigated. In laboratory condition, after the seedlings of Iris pseudacorus were submerged for 0, 10, 20, 30 and 40 days, the plant height, number of leaves, leaf area, number of roots and roots volume and other morphological indictors and the chlorophyll content, MDA, soluble sugar and starch of leaves were analyzed. The results indicated that after submerging for 40 days, the plant height, number of leaves, leaf area, number of roots and roots volume of submerged plant were respectively 11.53 cm, 4.00, 9.94 cm2, 5.33 and 0.18 cm3, which were lower than that of control plant significantly; the content of chlorophyll a, chlorophyll b and chlorophyll a+b significantly decreased to 0.26 mg/g, 0.10 mg/g and 0.35 mg/g, respectively; chlorophyll a/b, the content of MDA and soluble sugar significantly increased to 2.76, 0.0245 μmol/L and 95.11 mg/g, respectively; after submerging for 20 days, the starch content increased to the maximum (37.67 mg/g), then decreased to 34.56 mg/g after 40 days. In submergence condition, plant growth was inhibited, the chlorophyll content was degraded and membrane lipid peroxidation was intensified. To adapt to the energy demand under water, both the chlorophyll a/b ratio and the content of direct supply energy materials of the plant increased.