欢迎访问《中国农学通报》,

中国农学通报 ›› 2015, Vol. 31 ›› Issue (20): 1-4.doi: 10.11924/j.issn.1000-6850.casb15020115

• 畜牧 动物医学 蚕 蜂 •    下一篇

提取方法对饲用苎麻绿原酸和总黄酮提取效率的影响

刘俊欢1,杜晓华1,揭红东1,揭雨成1,2,邢虎成1,2   

  1. (1湖南农业大学苎麻研究所,长沙 410128;2湖南省作物种质创新与资源利用重点实验室,长沙 410128)
  • 收稿日期:2015-02-28 修回日期:2015-03-31 接受日期:2015-04-17 出版日期:2015-07-28 发布日期:2015-07-28
  • 通讯作者: 刘俊欢
  • 基金资助:
    国家科技平台项目“国家饲草与饲纤兼用作物种质资源平台”(2012-070;2013-070; 2014070);湖南省科技计划重点项目“苎麻抗逆基因的挖掘与应用”(2010TP4004-1; 2010FJ2013;2012FJ4063);国家科技支撑计划“环洞庭湖避灾种植模式与关键技术研究”(2012BAD20B05-04)。

Impact of Extraction Methods on Extraction Efficiency of Chlorogenic Acid and Flavonoids in Forage Ramie

Liu Junhuan1, Du Xiaohua1, Jie Hongdong1, Jie Yucheng1,2, Xing Hucheng1,2   

  1. (1Institute of Ramie, Hunan Agricultural University, Changsha 410128; 2Hunan Provincial Key Laboratory of Corp Germplasm Innovation and Utilization, Changsha 410128)
  • Received:2015-02-28 Revised:2015-03-31 Accepted:2015-04-17 Online:2015-07-28 Published:2015-07-28

摘要: 为了研究提取方法对饲用苎麻绿原酸和总黄酮提取效益的影响,笔者采用真空冷冻干燥和热风干燥2种不同的干燥提取方法,对‘湘饲苎X1’和宁远苎麻的各部位器官进行了绿原酸和总黄酮的定量提取。分析得率差异表明:无论是绿原酸还是总黄酮,‘湘饲苎X1’和宁远苎麻不同部位器官的测定含量,均表现为真空冷冻干燥样品明显高于热风干燥。在真空冷冻条件下,‘湘饲苎X1’的营养根、叶片、地下茎和茎的绿原酸测定含量提高幅度都大于100%,各器官总黄酮测定含量提高幅度也超过了1倍,且茎提高了6.08倍。宁远苎麻在真空冷冻干条件下,叶、地下茎、营养根、茎中绿原酸和总黄酮测定含量也都大幅提高。由此可见,在饲用苎麻药理成分含量的提取工艺上,真空冷冻干燥方式明显优于热风干燥方式。

关键词: 典型草原, 典型草原, 光合有效辐射, 太阳总辐射

Abstract: In order to study the impact of effective extraction method on extraction efficiency of chlorogenic acid and total flavonoids in ramie, the author extracted chlorogenic acid and total flavonoids of different parts of ramie varieties ‘Xiangsibi X1’ and Ningyuan quantitatively with the methods of vacuum freeze-drying extraction and hot air drying extraction. The results showed that the contents of chlorogenic acid and total flavonoids which were measured by vacuum freeze-drying extraction were higher than those by hot air drying extraction. Under the method of vacuum freeze-drying extraction, the chlorogenic acid content of nutritive roots, leaves, underground stems and stems increased more than 100%, and the total flavonoids content doubled that of hot air dried samples, the total flavonoids content of stems increased 6.08-fold especially. The chlorogenic acid and total flavonoids contents of Ningyuan ramie also substantially increased under vacuum freeze-drying extraction. Thus, when it comes to the extraction process of pharmacological components in forage ramie, the vacuum freeze-drying extraction was better than hot air drying extraction.