堆肥和枯草芽孢杆菌协同调控黄瓜幼苗生长的机制探究

doi:10.11924/j.issn.1000-6850.casb15040138

中国农学通报 ›› 2015, Vol. 31 ›› Issue (28): 71-78.doi: 10.11924/j.issn.1000-6850.casb15040138

所属专题：园艺

堆肥和枯草芽孢杆菌协同调控黄瓜幼苗生长的机制探究

毕延刚,田永强

(中国农业大学农学与生物技术学院,北京 100193）

收稿日期:2015-04-17 修回日期:2015-04-29 接受日期:2015-04-29 出版日期:2015-10-27 发布日期:2015-10-27
通讯作者: 田永强
基金资助:
国家自然科学基金“基于土壤-作物同步调控的设施连作黄瓜根际微生态平衡恢复机制研究”(31401916)；中央高校基本科研业务费专项资金项目“根际微生物多样性参与调控黄瓜生长的微生态学机制”(2014XJ035)。

Mechanism Exploration of the Coordinated Regulation Effects of

Compost and Bacillus subtilis on Cucumber Seedling Growth

Bi Yangang, Tian Yongqiang(College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193)

Received:2015-04-17 Revised:2015-04-29 Accepted:2015-04-29 Online:2015-10-27 Published:2015-10-27

摘要/Abstract

摘要： 土壤生物学障碍是制约中国设施蔬菜生产可持续发展的主要瓶颈之一，而土壤病原菌是引起土壤生物学障碍的重要因素之一。笔者以土壤生物学障碍问题突出的黄瓜为研究对象，探究了堆肥和枯草芽孢杆菌对黄瓜枯萎病病原菌及植株生长的影响。结果表明：堆肥中的微生物和枯草芽孢杆菌均可显著降低病原菌对黄瓜生长的抑制作用，且二者具有协同效应；堆肥微生物主要影响黄瓜幼苗一级侧根（直径0.3~0.9 mm）的绝对长度和二级侧根（直径0~0.3 mm）的相对量，而枯草芽孢杆菌接种主要影响了黄瓜幼苗一级侧根的绝对长度。综合分析认为，堆肥主要通过其微生物与枯草芽孢杆菌协同抵御黄瓜病原菌，并促进根系生长。

关键词: 蚜虫, 蚜虫, 基因组DNA, 提取方法, CTAB, 蛋白酶K

Abstract: Soil bio-obstacle is one of the main bottlenecks that restrict the sustainable development of greenhouse vegetable production in China, and soil-born pathogen is a major factor that induces soil bio-obstacle. Since soil bio-obstacle is common in cucumber production, the author investigated the effects of compost and Bacillus subtilis on the pathogen caused Fusarium wilt and plant growth. The results showed that both microorganisms in compost and Bacillus subtilis could significantly reduce the inhibition of pathogen to cucumber growth, and had synergistic effect. Microorganisms in compost mainly influenced the absolute length of the first lateral roots (0.3-0.9 mm diameter) and the relative amount of the second lateral roots (0-0.3 mm diameter), while Bacillus subtilis mainly influenced the absolute length of the first lateral roots. Based on these results, microorganisms in compost could cooperate with B. subtilis to inhibit cucumber pathogen and enhance cucumber root growth.

毕延刚,田永强. 堆肥和枯草芽孢杆菌协同调控黄瓜幼苗生长的机制探究[J]. 中国农学通报, 2015, 31(28): 71-78.

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堆肥和枯草芽孢杆菌协同调控黄瓜幼苗生长的机制探究

Mechanism Exploration of the Coordinated Regulation Effects of

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