玉米SAMS家族基因鉴定及其在逆境胁迫下的表达分析

doi:10.11924/j.issn.1000-6850.casb15090139

中国农学通报 ›› 2016, Vol. 32 ›› Issue (8): 30-36.doi: 10.11924/j.issn.1000-6850.casb15090139

所属专题：生物技术；玉米

玉米SAMS家族基因鉴定及其在逆境胁迫下的表达分析

余爱丽,赵晋锋,王高鸿,杜艳伟,李颜方,张正

(山西省农业科学院谷子研究所/特色杂粮种质资源发掘与育种山西省重点实验室,山西长治 046011）

收稿日期:2015-09-30 修回日期:2016-02-24 接受日期:2015-11-25 出版日期:2016-03-18 发布日期:2016-03-18
通讯作者: 赵晋锋
基金资助:
本研究由国家转基因生物新品种培育重大专项“抗纹枯病转基因育种新材料获得”(2011ZX08003-001)；山西省农业科学院博士研究基金“一个SAM合成通路中的变异对拟南芥甲硫氨酸积累和抗逆性的影响”(YBSJJ1306)；山西省农业科学院科技自主创新能力提升工程项目(2015ZZCX-09)。

Identification of SAMS Genes From Maize and Its Expression Under Adversity Stresses

Yu Aili, Zhao Jinfeng, Wang Gaohong, Du Yanwei, Li Yanfang, Zhang Zheng

(Millet Research Institute, Shanxi Academy of Agricultural Sciences/Shanxi Key Laboratory of Genetic Resources and Breeding in Minor Crops, Changzhi Shanxi 046011)

Received:2015-09-30 Revised:2016-02-24 Accepted:2015-11-25 Online:2016-03-18 Published:2016-03-18

摘要/Abstract

摘要： S-腺苷甲硫氨酸合成酶是植物代谢中的一个关键酶，它催化ATP与甲硫氨酸合成S-腺苷甲硫氨酸。利用生物信息学方法系统分析了玉米基因组中的4个S-腺苷甲硫氨酸合成酶(ZmSAMS)成员的参数特征，结果表明4个ZmSAMS成员参数非常接近。不同物种SAMS序列比对及进化分析显示植物SAMS基因在进化过程中具有非常保守的序列和结构。启动子顺势元件预测和蛋白功能分析显示ZmSAMS基因成员可能参与逆境应答、调控转录调控、信号转导、免疫应答等生理生化过程。随后的Real-time PCR验证了ZmSAMS家族基因成员参与了干旱、高温、低温、盐和ABA等逆境胁迫应答。因此推测玉米ZmSAMS基因可能在玉米的逆境胁迫应答中起一定作用。本研究为进一步阐明玉米ZmSAMS家族基因在逆境应答中的功能、机制提供了有益线索。

关键词: 芒果细菌性黑斑病, 芒果细菌性黑斑病, 野油菜黄单胞杆菌芒果致病变种, 生理生化特征

Abstract: S-adenosy lmethionine synthetase is a key enzyme in plant metabolism. It could catalyze ATP and methionine synthesis of S-adenosy lmethionine. In this study, the parameter characteristics of 4 S-adenosy lmethionine synthetase members (ZmSAMS) in maize genome were analyzed by bioinformatics methods. The results showed that the parameters of the 4 members were close to each other. Different SAMS sequence alignment and evolution analysis indicated that the SAMS gene had a conserved sequence and structure in the process of evolution. Promoter cis-elements prediction and protein function analysis showed that the ZmSAMS genes might be involved in the physiological and biochemical processes such as stress response, transcriptional regulation, signal transduction, immune response and so on. Real-time PCR validated that the ZmSAMS genes took part in the stress response of drought, high temperature, low temperature, salt, ABA and so on. So the authors speculated that the ZmSAMS genes played certain roles in different stress responses. This study provided useful clues for further elucidation of the function and mechanism of ZmSAMS genes in stress response.

余爱丽,赵晋锋,王高鸿,杜艳伟,李颜方,张正. 玉米SAMS家族基因鉴定及其在逆境胁迫下的表达分析[J]. 中国农学通报, 2016, 32(8): 30-36.

Yu Aili,Zhao Jinfeng,Wang Gaohong,Du Yanwei,Li Yanfang and Zhang Zheng. Identification of SAMS Genes From Maize and Its Expression Under Adversity Stresses[J]. Chinese Agricultural Science Bulletin, 2016, 32(8): 30-36.

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玉米SAMS家族基因鉴定及其在逆境胁迫下的表达分析

Identification of SAMS Genes From Maize and Its Expression Under Adversity Stresses

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