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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (26): 75-79.doi: 10.11924/j.issn.1000-6850.casb16030194

• 食品 营养 检测 安全 • 上一篇    下一篇

富硒酿酒酵母的选育及优化培养

李玉梅1,王根林2,刘峥宇3,李 艳1,孙 雷2,魏 丹1   

  1. (1黑龙江省农业科学院,土壤肥料与环境资源研究所,黑龙江省重点实验室,哈尔滨 150086；2黑龙江省农业科学院,畜牧研究所,哈尔滨 150086；3黑龙江省绥滨农场,黑龙江鹤岗 154213）
  • 收稿日期:2016-03-28 修回日期:2016-04-18 接受日期:2016-04-25 出版日期:2016-09-21 发布日期:2016-09-21
  • 通讯作者: 李玉梅
  • 基金资助:
    黑龙江省博士后科研启动基金“水稻对生物有机硒的富集和转化研究”(LBH-Q14148)；农业部产业体系项目“粮豆轮作土壤培肥及养分管理关键技术”(CARS-04-01A)。

Screening and Optimized Culture of Selenium-enriched Saccharomyces cerevisiae

Li Yumei1, Wang Genlin2, Liu Zhengyu3, Li Yan1, Sun Lei2, Wei Dan1   

  1. (1Institute of Soil Fertilizer and Environment Resources, Heilongjiang Academy of Agricultural Sciences, Heilongjiang Provincial Key Laboratory, Harbin 150086;2Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150086; 3SuiBin Farm in Heilongjiang Province, Hegang Harbin 154213)
  • Received:2016-03-28 Revised:2016-04-18 Accepted:2016-04-25 Online:2016-09-21 Published:2016-09-21

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摘要： 选育高富硒酵母菌株，优化发酵培养条件，以提高酵母生物富集、转化有机硒的能力，为富硒农牧业生产提供一种安全的有机硒源。通过紫外线诱变、抗性筛选试验，获得富硒能力强的酵母菌株，采用单因素及正交试验设计，优化培养基及发酵培养条件。研究结果表明：试验分离筛选得到一株可抗亚硒酸钠5 mg/mL、抗乙硫氨酸10 mg/kg的双抗菌株，经鉴定为酿酒酵母(Saccharomyces cerevisiae)。最佳发酵配方以葡萄糖、蛋白胨、酵母膏为主，添加少量无机盐。确定最优培养条件起始pH 4.0，装液量50 mL/500 mL，摇床转速160 r/min，种子液接种量为10% (V/V)，30℃培养28 h，在最优发酵培养条件下菌株富硒能力可达26.75 mg/g干菌体。

关键词: 兰科植物, 兰科植物, 开花时间, 花发育

Abstract: In order to increase the ability of bio-concentration and organic selenium transform of yeast, and provide safe organic selenium resource for the production of selenium-rich agriculture and animal husbandry, selenium-enriched yeast was screened and the culture condition was optimized. Selenium-enriched yeast strain was obtained through UV mutagenesis and resistance screening test, culture medium and ferment condition were optimized through single factor orthogonal test. The results showed that a strain which could resist 5 mg/mL sodium selenite and 10 mg/kg ethionine was isolated and identified as Saccharomyces cerevisiae. The optimum ferment formula consisted of glucose, peptone, yeast extract, adding a small amount of inorganic salt. The optimum culture medium condition was initial pH value of 4, liquid volume of 50 mL/500 mL, shaking speed of 160 r/min, seed inoculation volume of 10% (V/V), and cultured for 28 h at 30℃, the ability of selenium-enriched strain was up to 26.75 mg/g dry cell under the optimum condition.

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