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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (34): 123-128.doi: 10.11924/j.issn.1000-6850.casb16040132

所属专题： 园艺

• 生物技术科学 • 上一篇    下一篇

青花菜小孢子发育时期与花器形态的相关性

袁建民,木万福,杨 龙,李易蓉,但 忠,苏银玲,麻继仙,杨子祥   

  1. (云南省农业科学院热区生态农业研究所,云南元谋 651300）
  • 收稿日期:2016-04-21 修回日期:2016-06-02 接受日期:2016-06-20 出版日期:2016-12-15 发布日期:2016-12-15
  • 通讯作者: 木万福
  • 基金资助:
    云南省重点新产品开发“云南耐热花椰菜、温室专用型黄瓜等主要蔬菜新品种选育及产业化示范”(2014BB020)；云南省社会发展计划(2010BB013)；云南省农科院优秀团队培育及科研超前预研项目“花椰菜资源筛选、新品种选育及示范项目”(2014CZYY008)。

Correlation of Microspore Development Period and Flower Morphological Characteristics of Broccoli

Yuan Jianmin, Mu Wanfu, Yang Long, Li Yirong, Dan Zhong, Su Yinling, Ma Jixian, Yang Zixiang   

  1. (Research Institute of Tropical Eco-agricultural Sciences, Yunnan Academy of Agricultural Sciences, Yuanmou Yunnan 651300)
  • Received:2016-04-21 Revised:2016-06-02 Accepted:2016-06-20 Online:2016-12-15 Published:2016-12-15

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摘要： 对青花菜小孢子发育时期细胞学特征及其与花器外部形态的相关性展开研究，试图从花器的外部形态特征来推断小孢子的发育时期，最终目的是为花药或游离小孢子培养研究提供科学依据。结果表明，青花菜小孢子发育经历四分体时期、单核早中期、单核靠边期和双核期共4个时期，供试3个青花菜各时期细胞学特征存在明显差异。青花菜小孢子发育时期与花蕾大小和花药颜色等指标密切相关。供试青花菜花蕾纵径为10.52~11.05 mm，花蕾横径为3.64~4.25 mm，花药长度为2.85~2.87 mm，花药宽度为0.95~0.96 mm，且花瓣微露出花萼，花瓣和花药均为淡黄色时，80%以上的小孢子发育至单核靠边期。

关键词: 湖南棉花区域试验, 湖南棉花区域试验, GGE双标图, 鉴别力, 代表性

Abstract: The cytological characteristics of broccoli (Brassica oleracea var. italica) microspore development, bud morphology in different microspore development periods and anther color were observed to identify the period of microspore development directly from the morphological characteristics of buds and anther of broccoli, which would provide the scientific evidence for culturing of anther and isolated microspore. The results showed that broccoli microspore development was divided to four stages: tetrad period, early or mid-uninucleate period, late-uninucleate period, and binuclear period, and each stage had obvious characteristics. The microspore development period of broccoli was closely related with bud size and anther color. The buds were 10.52-11.05 mm in longitudinal diameter and 3.64-4.25 mm in transversal diameter, and the anthers 2.85-2.87 mm in length and 0.95-0.96 mm in width. The petals were calyx slightly and the anthers and the petals were yellowish. Eighty percent microspores could develop to the stage of late-uninucleate.