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中国农学通报 ›› 2016, Vol. 32 ›› Issue (27): 99-104.doi: 10.11924/j.issn.1000-6850.casb16060040

所属专题: 食用菌 食用菌

• 农学 农业基础科学 • 上一篇    下一篇

金针菇生物降解与染料脱色作用研究

李 哲,艾柳英,李钊锋,郑 静,吕书仪,孙淑静   

  1. 福建农林大学生命科学学院,福建农林大学生命科学学院,福建农林大学生命科学学院,福建农林大学生命科学学院,福建农林大学生命科学学院,福建农林大学生命科学学院
  • 收稿日期:2016-06-07 修回日期:2016-08-23 接受日期:2016-07-22 出版日期:2016-09-29 发布日期:2016-09-29
  • 通讯作者: 艾柳英
  • 基金资助:
    财政部、农业部重大农技推广项目“福建省食用菌产业重大农技推广服务体系建设”(KNJ15301C);福建省科技厅科技创新平台建设项目“福建省食用菌产业重大研发平台”(2014N2101)。

Biodegradation and Decolorization Effect of Flammulina velutipers

  • Received:2016-06-07 Revised:2016-08-23 Accepted:2016-07-22 Online:2016-09-29 Published:2016-09-29

摘要: 为了解金针菇对染料的降解作用机制,研究测定了金针菇发酵液木质素降解酶系酶活,探究不同处理发酵液对3种化合染料的脱色影响。结果表明,液体培养条件下,化合染料的降解既可通过金针菇发酵液中的菌丝吸附,又能利用其分泌的木质素降解酶系转化。对化合染料起转化作用的并不是漆酶,而以菌体吸附为主,菌体对不同化合染料的吸附有一定选择性;已灭活的菌体与未灭活的菌体对染料的吸附效果也不同,表明温度可以改变菌体表面结构,使其更易吸附溶液中的染料。该研究对当前染料的处理具有重要应用价值。

关键词: 盐爪爪群落, 盐爪爪群落, 芦苇群落, 季相变化, 民勤, 盐渍荒漠草地

Abstract: To explore the degradation mechanism of dyes, the ligninolytic enzyme system was investigated in detail by analyzing the enzyme activity of laccase, manganese peroxidase (MnP) and lignin peroxidase (LiP). At the same time, the decolorization effects on three kinds of chemical dye to fermentation liquid in different treatments were studied. The results showed that under liquid culture condition, the dyes could both be adsorbed by mycelium and transformed by the ligninolytic enzyme system which was secreted by Flammulina velutipes fermentation. However, the key substances to chemical dyes’ transformation was not laccase but mycelium adsorption, and the adsorption to different chemical dyes had certain selectivity. The adsorption effect of the dyes by the inactivated mycelia and the non killed mycelia was also different, which suggested that the temperature could change the surface structure of the cell and make it easier to adsorb the dyes in the solution. This study had essential application value for the current treatment of dyes.

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