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中国农学通报 ›› 2016, Vol. 32 ›› Issue (31): 123-127.doi: 10.11924/j.issn.1000-6850.casb16070014

所属专题: 烟草种植与生产

• 植物保护 农药 • 上一篇    下一篇

海藻酸对烟草花叶病毒的抑制作用研究

陈芊伊1,郭 尧2,石永春1   

  1. (1河南农业大学生命科学院,郑州 450002;2郑州外国语新枫杨学校,郑州 450002)
  • 收稿日期:2016-07-04 修回日期:2016-10-12 接受日期:2016-08-22 出版日期:2016-11-10 发布日期:2016-11-10
  • 通讯作者: 石永春
  • 基金资助:
    河南省烟草公司项目“质膜保护剂对烟草花叶病抗性的影响及应用技术研究”(HYKJ201409)。

The Inhibition Mechanism of Alginate to Tobacco Mosaic Virus

Chen Qianyi1, Guo Yao2, Shi Yongchun1   

  1. (1Life Science College, Henan Agricultural University, Zhengzhou 450002;2New Fengyang Campus, Zhengzhou Foreign Language School, Zhengzhou 450002)
  • Received:2016-07-04 Revised:2016-10-12 Accepted:2016-08-22 Online:2016-11-10 Published:2016-11-10

摘要: 为分析海藻酸对烟草花叶病毒的抑制机理,研究海藻酸对烟草花叶病毒的体外钝化和对烟草系统抗性的诱导效果。结果发现,叶面喷施海藻酸后,枯斑抑制率可达到66.67%,复制增殖抑制率可达到34.67%,烟草N基因表达水平增加2.67倍,过氧化物酶活性增加3.46倍,超氧化物歧化酶活性增加1.43倍,认为海藻酸可能通过增强烟草抗病和抗氧化能力,抑制烟草花叶病病毒在烟草中的增殖。

关键词: 淫羊藿, 淫羊藿, 生态适宜性, 种植区划

Abstract: To understand the inhibition mechanism of alginate to tobacco mosaic virus (TMV), the in vitro inactivation and systemic resistance induction were investigated in tobacco treated with alginate. The results showed that, after spraying alginate, the lesion inhibition rate reached 66.67%, proliferation inhibition rate reached 34.67%, the expression level of N gene increased to 2.67 times of the control levels, POD activity increased to 3.46 times, SOD activity increased to 1.43 times of the control levels. Therefore, proliferation inhibition of alginate to TMV might be caused via the increase of disease-resistance and oxidation resistance abilities.