关键词: β-罗勒烯, β-罗勒烯, PR1启动子, PDF1.2启动子, 荧光素酶基因, 活体荧光检测系统

Abstract: To clarify the effect of growing-microbial agents on paddy bioremediation and fertilization, this study set growing- microbial agent “chenaorunze” as representative material, five treatments including 0 kg/hm2 (CK), 30 kg/hm2, 60 kg/hm2, 90 kg/hm2, 120 kg/hm2 were set to research the effects of growingmicrobial agents on topsoil nutrients, heavy metal Cd and yield of double cropping rice. The results showed that: rice yield showed an increasing trend with increasing dosage of“chenaorunze”, the effective panicle and grains per spike were the major factors of high- yield;“chenaorunze” improved soil available nutrient effectively, increased soil supplying nutrient capability; heightened the nitrogen and potassium content of root, promoted nitrogen and potassium in stem transferring to grain, and the capacity of lodging- resistant was strengthened as the addition of potassium content in stem;“chenaorunze”could reduce rice Cd accumulation mainly by the way of decreasing soil effective cadmium, and Cd accumulation showed a decreasing trend with increasing dosage of“chenaorunze”, thus, it was beneficial to guarantee the safe production of double cropping rice. The optimum dosage of“chenaorunze”was about 90 kg/hm2 with a better bioremediation and fertilization effect from the perspective of decreasing cost and increasing efficiency, the yield of early and late rice increased by 12.9% and 12.6% compared with control respectively.