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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (16): 65-70.doi: 10.11924/j.issn.1000-6850.casb16100007

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

外源H202对百合小鳞茎生根发芽和内源激素含量的影响

孙亮,王有国,贾文杰   

  1. 云南农业大学园林园艺学院,云南农业大学园林园艺学院,云南省农科院花卉研究所
  • 收稿日期:2016-10-04 修回日期:2016-12-13 接受日期:2016-12-16 出版日期:2017-06-08 发布日期:2017-06-08
  • 通讯作者: 王有国
  • 基金资助:
    云南省教育厅科学研究基金重点项目“NO等三种生物活性小分子物质对百合试管生小鳞茎休眠的影响”(A3007941)。

Effects of Exogenous H2O2 on Rooting and Germination and Endogenous Hormone Contents of Lily Bulb

  • Received:2016-10-04 Revised:2016-12-13 Accepted:2016-12-16 Online:2017-06-08 Published:2017-06-08

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摘要： 拟探究外源H202对西伯利亚百合小鳞茎生根发芽的影响和对内源激素含量的调节,分别用0、0.5%、3%、5%、10%五种不同浓度梯度的双氧水(H202水溶液)及0.5mmol/LDMTU(外源H202抑制剂)分别处理百合鳞片生小鳞茎,种植30d后统计分析。结果表明：5%浓度梯度双氧水处理下的百合小鳞茎生根发芽效果最好；0.5mmol/LDMTU(外源H202抑制剂)处理效果最不理想；在百合小鳞茎生根发芽过程中内源激素脱落酸(ABA)的含量呈降低趋势,而赤霉素(GA)和生长素(IAA)的含量不断升高,且变化幅度较对照明显。

关键词: 蜂蜜, 蜂蜜, 定价, 保健功能食品

Abstract: To study the effects of exogenous H2O2 on germination and regulation of endogenous hormone contents of‘Siberia’lily, the authors used five different concentration gradients of hydrogen peroxide solution (H2O2 aqueous solution), which were 0, 0.5%, 3%, 5%, 10%, respectively, and 0.5 mmol/L DMTU (exogenous H2O2 scavenger) to treat the small lily bulbs 30 days. The results showed that 5% H2O2 had a great influence on the rooting and sprouting of lily bulbs, the effect of 0.5 mmol/L DMTU treatment was not ideal compared with 5% H2O2; the ABA content decreased, while the GA and IAA contents increased, and had a more significant variation than control during the rooting and sprouting of lily bulbs. The suitable concentration of exogenous H2O2 could be beneficial to lily bulbs’germination.