拟南芥与烟草细胞低温胁迫下的粘弹性变化规律与耐冷性比较

doi:10.11924/j.issn.1000-6850.casb17030159

中国农学通报 ›› 2017, Vol. 33 ›› Issue (17): 19-23.doi: 10.11924/j.issn.1000-6850.casb17030159

所属专题：生物技术；烟草种植与生产

拟南芥与烟草细胞低温胁迫下的粘弹性变化规律与耐冷性比较

邓君^1,2,周铁安^1,2,胡家金^1,2,陈宗星^1,2,谢杰^1,2

(¹湖南农业大学生物科学技术学院,长沙 410128；²湖南农业大学细胞力学与生物传感研究所,长沙 410128）

收稿日期:2017-03-24 修回日期:2017-04-20 接受日期:2017-04-21 出版日期:2017-06-16 发布日期:2017-06-16
通讯作者: 周铁安
基金资助:
湖南省教育厅委托项目“模式植物拟南芥细胞动态力学性能的测定与研究”(12W004)；湖南省科技厅重点项目“多通道细胞力学分析平台关键技术的研究”(2013T1009)。

Viscoelastic Changes and Cold Tolerance Comparison of Arabidopsis and Tobacco Cells Under Low Temperature Stress

Deng Jun^1,2, Zhou Tiean^1,2, Hu Jiajin^1,2, Chen Zongxing^1,2, Xie Jie^1,2

(¹College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128; ²Cell Mechanic and Biosensing Institute, Hunan Agricultural University, Changsha 410128)

Received:2017-03-24 Revised:2017-04-20 Accepted:2017-04-21 Online:2017-06-16 Published:2017-06-16

摘要/Abstract

摘要： 研究旨在通过实时监测与比较拟南芥与烟草BY-2细胞在降温过程的粘弹性变化规律以建立衡量植物耐冷性的一种新指标。采用修饰带正电荷的聚二烯丙基二甲基氯化铵石英晶体微天平实时跟踪测定拟南芥悬浮细胞和烟草BY-2细胞在降温过程的粘弹性变化规律。随着温度的降低，拟南芥和烟草细胞的粘弹性指数绝对值均下降，细胞硬度增加。温度降得越低，粘弹性指数绝对值下降越大，细胞越硬。当温度从25℃降到5℃时，拟南芥细胞比BY-2细胞的粘弹性指数变化值要小，这与拟南芥比烟草耐受低温相符。植物细胞在低温下的粘弹性变化规律可以用来研究植物寒冷胁迫和作为衡量植物耐冷性的一种新指标。

关键词: 土地经济, 土地经济, 征地补偿, 文献法, 对比分析法, 公共利益, 发展权, 社会保障

Abstract: Positively charged poly(diallyldimethylammonium chloride) (PDADMAC)modified quartz crystal microbalance (QCM) was used for the real-time monitoring of the viscoelastic changes of Arabidopsis suspension cells and BY-2 cells when they were subjected to low temperature stress to compare cold tolerance of these two types of plant cells. The results showed that as the decrease of the temperature, the cell viscoelastic index (CVI) of both types of the cells decreased and the cells became harder, the lower the temperature, the larger the drop in CVI. Over the temperature drop range (25°C-5°C), the change in CVI for Arabidopsis cells is smaller than that of BY-2 cells, which is consistent with that Arabidopsis has better cold tolerance than that of Nicotiana tabacumL. Therefore, we propose to use CVI as a new parameter to indicate the cold tolerance of plant cells.

邓君,周铁安,胡家金,陈宗星,谢杰. 拟南芥与烟草细胞低温胁迫下的粘弹性变化规律与耐冷性比较[J]. 中国农学通报, 2017, 33(17): 19-23.

Deng Jun,Zhou Tiean,Hu Jiajin,Chen Zongxing and Xie Jie. Viscoelastic Changes and Cold Tolerance Comparison of Arabidopsis and Tobacco Cells Under Low Temperature Stress[J]. Chinese Agricultural Science Bulletin, 2017, 33(17): 19-23.

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拟南芥与烟草细胞低温胁迫下的粘弹性变化规律与耐冷性比较

Viscoelastic Changes and Cold Tolerance Comparison of Arabidopsis and Tobacco Cells Under Low Temperature Stress

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