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中国农学通报 ›› 2018, Vol. 34 ›› Issue (24): 46-49.doi: 10.11924/j.issn.1000-6850.casb17060115

• 生物技术科学 • 上一篇    下一篇

四环素及盐酸四环素对草履虫的毒性效应研究

张辰笈,丁 霜,赵 也,陈 瑛   

  1. 哈尔滨师范大学生命科学与技术学院,哈尔滨师范大学生命科学与技术学院,哈尔滨师范大学生命科学与技术学院,哈尔滨师范大学生命科学与技术学院
  • 收稿日期:2017-06-26 修回日期:2018-07-07 接受日期:2017-08-24 出版日期:2018-08-28 发布日期:2018-08-28
  • 通讯作者: 陈 瑛
  • 基金资助:
    国家自然科学基金“几种纤毛虫皮层类中间纤维系统的研究”(31471950);黑龙江省博士后基金“纤毛虫皮层类中间纤维系统及其它超微 结构的毒理学研究”。

Toxic Effects on Paramecium caudatum: Tetracycline and Tetracycline Hydrochloride

陈 瑛   

  • Received:2017-06-26 Revised:2018-07-07 Accepted:2017-08-24 Online:2018-08-28 Published:2018-08-28

摘要: [目的]本研究旨在探讨四环素及盐酸四环素对草履虫的急慢性毒性效应,初步评价四环素类抗生素对水生生物的潜在威胁。[方法]测定了四环素和盐酸四环素24h半数致死浓度(LC50)和最低有效浓度(LOEC),并以LOEC进行胁迫实验,观察草履虫15天单克隆生长曲线变化。[结果] 结果表明:四环素对尾草履虫的LC50值为52.36125mg.L-1,LOEC值为15.19917mg.L-1;盐酸四环素对尾草履虫的LC50值为50.24583mg.L-1,LOEC值为24.67175mg.L-1。15天慢性毒性试验显示两种抗生素对尾草履虫的单克隆生长曲线产生了显著抑制作用,使其种群增长率降低,种群数量减少。[结论]说明四环素和盐酸四环素对单细胞动物存在急慢性毒性效应,应明确其使用和排放标准,并积极开展监测和污染防控工作。

关键词: AWTP指数, AWTP指数, EDP指数, 标准化变量, 降水量距平百分率

Abstract: The aim of this research is to discuss the acute and chronic toxic effects of tetracycline (TC) and tetracycline hydrochloride (HTC) on Paramecium caudatum , and preliminarily assess the potential threat of tetracycline antibiotics to aquatic organisms. 24h- lethal concentration 50% (LC 50) and the lowest observed effect concentration (LOEC ) of TC and HTC onParamecium caudatum were tested.LOEC was used in the stress test. 15-day monoclonal growth curve ofParamecium caudatum was investigated. The results were as follows: 24 h-LC 50 of TC was 52.36125 mg/L andLOEC was 15.19917 mg/L. 24 h-LC 50 of HTC was 50.24583 mg/L, LOEC value was 24.67175 mg/L. 15- day chronic toxic test showed that the two kinds of antibiotics had significant inhibitory effects on 15-day monoclonal growth curve ofParamecium caudatum . The increase rate of population declined and the numbers of individuals reduced. These results suggested that tetracycline and tetracycline hydrochloride had acute and chronic toxicity on single-cell animal. We should make a definite standard for using and emission, and monitor and control the pollution of TC and HTC.