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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (26): 48-51.doi: 10.11924/j.issn.1000-6850.casb17070111

所属专题： 生物技术； 油料作物

• 生物技术科学 • 上一篇    下一篇

大豆7S球蛋白β-伴大豆球蛋白亚基的氨基酸序列分析

舒文涛,耿 臻,李金花,李 琼,杨青春,张东辉,张保亮   

  1. 周口市农业科学院,周口市农业科学院,周口市农业科学院,周口市农业科学院,周口市农业科学院,周口市农业科学院,周口市农业科学院
  • 收稿日期:2017-07-21 修回日期:2017-09-12 接受日期:2017-09-21 出版日期:2018-09-19 发布日期:2018-09-19
  • 通讯作者: 杨青春
  • 基金资助:
    无基金

Amino Acid Sequence Analysis of 7s Globulin (β-conglycinin) of Soybean

杨青春, and 张保亮   

  • Received:2017-07-21 Revised:2017-09-12 Accepted:2017-09-21 Online:2018-09-19 Published:2018-09-19

摘要： 为了分析大豆7S球蛋白β-伴大豆球蛋白不同亚基氨基酸组成对大豆营养品质的影响，利用生物信息学软件(BIOEDIT，DNAMAN)对已在GenBank数据库中注册的大豆7S球蛋白β-伴大豆球蛋白亚基氨基酸序列分析各亚基氨基酸组成和氨基酸序列比对。结果表明，3种亚基中色氨酸、蛋氨酸、半胱氨酸含量较低，其中β-亚基则不含色氨酸；3种亚基富含谷氨酸和亮氨酸，其中α-亚基谷氨酸最高，为13.16%，β-亚基中亮氨酸的含量最高，为10.48%。谷氨酰胺、精氨酸、丝氨酸的含量也较高。氨基酸序列比对分析结果表明，序列保守位点较多，同源进化树分析证明3种亚基亲缘关系较近。

Abstract: To study the effect of amino acid composition of different subunits of 7s globulin (β -conglycinin) on the nutritional quality of soybean, the amino acid sequence compositions and comparison of 7s globulin (β -conglycinin) subunit of soybean submitted to GenBank database were studied by means of bioinformatics software (BIOEDIT and DNAMAN). The results showed that the contents of tryptophan, methionine and cystine in the three subunits were lower, and theβ- subunit was free of tryptophan. The three subunits were rich in glutamic acid and leucine, the content of glutamic acid was the highest inα -subunit, which was 13.16%, and the content of leucine in β- subunit was the highest, which was 10.48% . Glutamine, arginine and serine content were also relatively high. Amino acid sequence alignment analysis showed that there were more conserved sequences, and the homologous tree showed that the genetic relationship among the three subunits was close.