Abstract: To provide scientific management methods of water and fertilizer application, the effects of reducing fertilizer application on yield, output, quality, nutrient uptake and nitrogen utilization efficiency of greenhouse cultivation tomato under integration of water and fertilizer were studied in view of the problems such as excessive and unfavorable fertilization in Wuhu County, Anhui Province. The results showed that: compared with the conventional management system of water and fertilizer, the reduction of 20%-40% N or 20% N, P, K respectively under integration of water and fertilizer could increase yield, output and profit of tomato. There were many influence factors on tomato quality, under this soil fertility condition and fertilization rate, soluble sugar and total soluble solid content decreased, Vc content increased, proper reduction of fertilizer rate was beneficial to increasing the content of lycopene, too much reduction of fertilizer rate decreased lycopene content; reducing fertilizer application under integration of water and fertilizer could increase N fertilizer use efficiency. Considering tomato yield, quality and nitrogen utilization efficiency, the appropriate rates of N, P2O5, K2O were 240, 144, 240 kg/hm2 under this soil fertility condition and fertilization rate, respectively. The authors pointed out that although optimum fertilization, reducing fertilizer application under integration of water and fertilizer could enhance nitrogen use efficiency, the nitrogen use efficiency of greenhouse cultivation tomato was still very low, there was still certain potential to improve the utilization rate of nitrogen.