Abstract: Three major rice varieties of Sanjiang plain,‘Longjing 29’,‘Longjing 31’and‘Longjing 46’are chosen as materials and treated under low temperature at the heading and filling stages with artificial climate box in order to reveal the effect of low temperature on rice quality during mid and late growth period by analyzing rice appearance quality, nutritional quality and RVA profile parameter. The results indicated that: low temperature treatment at the heading and late filling stage greatly affected rice appearance quality; the protein content increased significantly at each growth stage while RVA primary parameter decreased; amylose content and RVA secondary parameter went upwards and downwards respectively on account of genotypic differences. Among all the characters studied, 17 pairs had extremely significant correlations and 5 pairs had significant correlations. The peak viscosity had the greatest correlation with 6 characters, and chalky rice rate had correlations with 5 characters. The results also showed that every character had correlation with two or above two characters. Based on the effect of low temperature on rice quality, this experiment provides references not only for rice quality certification in Sanjiang plain, but also for rice cultivation and resistance breeding.