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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (1): 134-143.doi: 10.11924/j.issn.1000-6850.casb17100070

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

HPLC-ELSD 法测定啤酒糟酶解物中低聚木糖

邓元元1, 胡超2, 兰时乐1   

  1. 1.湖南省长沙市马坡岭东湖农大路1号湖南农业大学生物科学技术学院;2.湖南农业大学生物科学技术学院
  • 收稿日期:2017-10-21 修回日期:2018-12-04 接受日期:2018-01-24 出版日期:2019-01-02 发布日期:2019-01-02
  • 通讯作者: 兰时乐
  • 基金资助:
    湖南省重点研发项目“池塘养殖水质净化新型微生态制剂研究与开发”(2016NK2103)。

The Determination of Xylooligosaccharide in Brewer''s Grains Hydrolysate Based on HPLC-ELSD Method

  • Received:2017-10-21 Revised:2018-12-04 Accepted:2018-01-24 Online:2019-01-02 Published:2019-01-02

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摘要： 为建立啤酒糟酶解物中低聚木糖HPLC-ELSD检测方法，将啤酒糟酶解物用纯水提取，色谱柱为COSMOSIL Sugar-D分析柱(250 mm×4.6 mm，5 μm)，流动相为乙腈-水(69:31，V/V)，流速为0.9 mL/min，柱温40℃，进样量3 μL，Evaporator Temperature 为78℃，载气流速为1.6 L/min，载气压力为0.5 MPa，采用峰面积外标法定量检测低聚木糖含量。结果表明，木二糖~木五糖分别在0.024~0.245 mg/mL质量浓度范围内呈良好的线性关系，检出限分别为0.0245、0.0245、0.02425、0.024 mg/mL，定量限分别为0.0907、0.0907、0.08981、0.8889 mg/mL，RSD 分别为0.203%、0.224%、0.233%、0.252%，样品加标回收率为97.31%~99.83%。该方法具有灵敏度高、重复性好等优点，可用于啤酒糟酶解物中低聚木糖的检测。

Abstract: The objective is to establish the HPLC-ELSD detection method of xylooligosaccharide in brewer''s enzymatic hydrolysis. Brewer''s grains hydrolysate was extracted with pure water, chromatographic column was COSMOSIL Sugar-Danalytical column (250 mm×4.6mm, 5 μm), the mobile phase was acetonitrile-water (69: 31, V/V), the flow rate was 0.9 mL/min, the column temperature was 40℃, the injection volume was 3 μL, the evaporator temperature was 78℃, flow rate of carrier gas was 1.6 L/min and carrier gas pressure was 0.5 MPa, and the content of xylooligosaccharide was determined by using the peak area external standard method. The results showed that Xylobiose-Xylopentaose had good linear relation in the range of 0.024-0.245 mg/mL, the detection limits were 0.0245, 0.0245, 0.02425, 0.024 mg/mL, and the limits of quantification were 0.0907, 0.0907, 0.08981, 0.8889 mg/mL. The relative standard deviations (RSD) were 0.203%, 0.224%, 0.233% and 0.252% and the spiked recoveries rates were 97.31%-99.83%. The results indicated that this method had a high sensitivity and good repeatability and could be applied to detect xylooligosaccharide in brewer''s grains.