Abstract: The paper aims to select potato varieties (lines) suitable for planting in saline soil. The experimental materials are 6 potato varieties (lines) from different regions. Under the condition of plant tissue culture, the authors conducted preliminary identification and screening of the potato varieties (lines) with salt tolerance through comparing the effects of salt stress on the appearance and physiological characteristics of plants by using different concentrations of NaCl to accumulate saline environment. The results showed that: the morphological indexes such as survival rate, rooting rate, rooting number, root length, number of leaves, height of plant, thick stem, etc. and the physiological indexes such as chlorophyll content, root vitality, proline content etc. of‘Dongnong 09-33069’were better than those of the other 5 test varieties (lines); moreover, the other 5 varieties did not appeared well on some indicators and had a poor resistance to salt stress. Thus,‘Dongnong 09-33069’has a strong resistance to stress and tolerance as well as a high stability in 6 varieties (lines), so it could be preliminarily identified as a salt-tolerant variety (line).