14-3-3蛋白结构与功能预测及其在木薯成熟期的表达分析

doi:10.11924/j.issn.1000-6850.casb18070142

中国农学通报 ›› 2018, Vol. 34 ›› Issue (36): 49-57.doi: 10.11924/j.issn.1000-6850.casb18070142

所属专题：生物技术；马铃薯

14-3-3蛋白结构与功能预测及其在木薯成熟期的表达分析

潘冉冉¹, 秦于玲¹, 乔景娟¹, 赖杭桂², 陈松笔¹

1.中国热带农业科学院热带作物品种资源研究所农业部木薯种质资源保护与利用重点实验室;2.海南大学热带农林学院

收稿日期:2018-07-31 修回日期:2018-09-20 接受日期:2018-09-25 出版日期:2018-12-24 发布日期:2018-12-24
通讯作者: 陈松笔
基金资助:
NSFC-CGIAR国际合作重点项目“木薯种质资源转录组与蛋白质组分子评价及品种高效选育研究”(31361140366)；国家科技支撑项目“高淀粉能源植物品种选育、栽培及液体生物燃料关键技术与示范”(2015BAD15B01)；海南省研究生创新科研课题“14-3-3 蛋白在木薯嫁接提高产量中的作用机理研究”(Hys2018-14)；国家自然基金项目“木薯有性四倍体遗传稳定性及其产量优势的遗传调控机理研究”(NO.31760408)。第一作者简介：潘冉冉，女，1992 年出生，山东济宁人，研究生，硕士，研究方向：木薯蛋白质组学辅助育种。通信地址：570228 海南大学热带农林学院，E-mail：489362685@qq.com。

The Structure, Function Prediction of 14-3-3 Protein and Its Expression at Maturity Stage of Cassava

Received:2018-07-31 Revised:2018-09-20 Accepted:2018-09-25 Online:2018-12-24 Published:2018-12-24

摘要/Abstract

摘要： 为了深入研究木薯14-3-3蛋白结构和功能，也为研究木薯块根淀粉积累的分子调控提供新思路。本研究采用生物信息学方法对木薯14-3-3蛋白家族的组成成分与理化性质、系统发育进化、导肽、亚细胞定位、二级结构、功能结构域、三级结构及亲水性、疏水性进行分析和预测，并对14-3-3基因在不同木薯品种中进行差异表达分析。结果表明：（1）木薯14-3-3蛋白家族16个异构体存在多个不同的保守结构域，二级结构相似的亲水性蛋白家族成员在进化关系上可划分为2大不同的类别；（2）14-3-3基因家族16个成员在花叶木薯、ZM-Seaside和华南9号(SC9)块根成熟期的表达水平存在显著性差异(P<0.05)，与花叶木薯和SC9相比，产量较高的木薯品系ZM-Seaside块根中14-3-3基因表达水平显著下降(P<0.05)，推测14-3-3蛋白在木薯块根成熟期的淀粉积累过程中可能起着负调控作用。

关键词: 重组蛋白、Western blot、免疫原性, 重组蛋白、Western blot、免疫原性

Abstract: This study aims to lucubrate our research on structures and functions of 14-3-3 protein family, as well as to provid a new insight into the molecular regulation of starch accumulation in cassava tuberous roots.The 14-3-3 proteins are signal transduction factors in plants, which play an important role in regulating starch synthesis in cassava tuberous roots. In the present study, the sequences composition, physicochemical properties, phylogenetic evolution, leader peptides, subcellular localization, the secondary structures, functional domains, tertiary structures and hydrophobicity/hydrophilicity of cassava 14-3-3 protein family were predicted and analyzed using bioinformatics tools. Differential expressions of 14-3-3 genes in different cassava cultivars were analyzed. The result showed that (1) cassava 14-3-3 protein family consisting of 16 isoforms had multiple conservative structure domains and these hydrophilic proteins, which are similar in the secondary structure, were classified into two different categories based on their evolutionary relationship, (2) there were significant differences in the expressions among 16 members from 14-3-3 gene family in the tuberous roots of the mature stage of mosaic cassava, ZM-Seaside and SC9 (P<0.05), and the expression of 14-3-3 gene family in cassava variety ZM-Seaside with high yield decreased significantly (p<0.05) compared to mosaic cassava and SC9 (P<0.05), inferring the 14-3-3 proteins may play a negative-regulation role in starch accumulation of the mature stage of cassava tuberous roots.

潘冉冉,秦于玲,乔景娟,赖杭桂,陈松笔. 14-3-3蛋白结构与功能预测及其在木薯成熟期的表达分析[J]. 中国农学通报, 2018, 34(36): 49-57.

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14-3-3蛋白结构与功能预测及其在木薯成熟期的表达分析

The Structure, Function Prediction of 14-3-3 Protein and Its Expression at Maturity Stage of Cassava

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