响应面优化绿色木霉产胶霉菌素培养基

doi:10.11924/j.issn.1000-6850.casb18080086

中国农学通报 ›› 2019, Vol. 35 ›› Issue (22): 42-46.doi: 10.11924/j.issn.1000-6850.casb18080086

响应面优化绿色木霉产胶霉菌素培养基

潘冬梅, 杨传伦, 张心青, 杨丹丹, 韩立霞, 刘海玉

黄河三角洲京博化工研究院有限公司

收稿日期:2018-08-22 修回日期:2019-03-22 接受日期:2019-03-25 出版日期:2019-08-13 发布日期:2019-08-13
通讯作者: 潘冬梅
基金资助:
山东省科技发展项目“木霉代谢产物胶霉菌素生产的关键技术研究”(No.2012YD21024)。

Response Surface: Optimizing the Medium for Gliotoxin Produced by Trichoderma viride

韩立霞, 刘海玉

Received:2018-08-22 Revised:2019-03-22 Accepted:2019-03-25 Online:2019-08-13 Published:2019-08-13

摘要/Abstract

摘要： 采用响应面法旨在优化绿色木霉产胶霉菌素培养基。试验选用Min Run Res IV设计对基础培养基的6个影响因子的效应进行评价，筛选显著影响因子，利用最陡爬坡试验确定其取值中心、中心组合试验确定其最佳取值，从而获得优化培养基。Min Run Res IV试验筛选出4个显著性影响因子：葡萄糖、豆粕、磷酸二氢钾和硫酸锌，经过最陡爬坡和中心组合试验，响应面分析得到最佳培养基组合为：葡萄糖26.54 g/L、酵母粉10 g/L、豆粕15.72 g/L、磷酸二氢钾0.452 g/L和硫酸锌0.0582 g/L、硫酸亚铁0.1 g/L，在最佳培养基下胶霉菌素效价较优化前提高了59.71%。

关键词: 蕉岭, 蕉岭, 福寿螺调查, 油茶粕

Abstract: Response surface method was used to optimize the culture medium for gliotoxin production by Trichoderma viride. Evaluation of the effects of the 6 factors relating to the basic medium was carried out through Min Run Res IV design, which revealed the significant influence factors. Steepest ascent design(SAD) and central composite design(CCD) were applied to determine the midpoint and the optimal value respectively, and the optimized culture medium was obtained. Glucose, soymeal, KH2PO4 and ZnSO4 were the most significant influence factors screened by Min Run Res IV. Response surface analysis of the SAD and CCD indicated that the optimal culture medium was composed of 26.54 g/L glucose,10 g/L yeast powder,15.72 g/L soymeal, 0.452g/L KH2PO4, 0.0582g/L ZnSO4 and 0.1g/L FeSO4, which resulted in a 59.71% increase of the titer of gliotoxin compared with the original medium.

中图分类号:

Q815

潘冬梅,杨传伦,张心青,杨丹丹,韩立霞,刘海玉. 响应面优化绿色木霉产胶霉菌素培养基[J]. 中国农学通报, 2019, 35(22): 42-46.

韩立霞 and 刘海玉. Response Surface: Optimizing the Medium for Gliotoxin Produced by Trichoderma viride[J]. Chinese Agricultural Science Bulletin, 2019, 35(22): 42-46.

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响应面优化绿色木霉产胶霉菌素培养基

Response Surface: Optimizing the Medium for Gliotoxin Produced by Trichoderma viride

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