稳定性同位素在植物次生代谢中的研究进展

doi:10.11924/j.issn.1000-6850.casb18110067

摘要/Abstract

摘要： 植物次生代谢是植物在长期的进化过程中与环境相互作用的结果，其产物在植物生命活动的许多方面起着重要的生理作用，同时也是药物、香料和工业原料的重要来源。早期有关植物次生代谢路径的研究主要是通过放射性同位素示踪技术完成的。本文就稳定性同位素发现、理化特性、示踪原理和特点，及其在植物次生代谢路径研究中的应用进展进行了总结，分析了该技术对已知植物次生代谢路径的修正和未知代谢路径研究等方面的贡献、并提出了未来稳定性同位素示踪技术在植物次生代谢物合成路径领域的研究方向和前景。

关键词: 甘肃, 甘肃, 全膜, 双垄沟播, 增产效果

Abstract: The secondary metabolism of plants is the result of the interaction between plants and the environment in the long process of evolution. The secondary metabolites products play an important physiological role in many aspects of plant life activities, and are also an important source of drugs, spices and industrial materials. Early studies of secondary metabolic pathways in plants have been carried out mainly through radioisotope tracer technology. This paper reviews the discovery, physicochemical properties, the principle and the application progress of stable isotope as tracer in plant secondary metabolic study. The application of stable isotope tracer technique to the modification of known secondary metabolic pathways of plants and the study of unknown metabolic pathways are analyzed. Besides, the direction and prospect of the stable isotope tracer technology in future study on plant secondary metabolic pathway is also presented.

雷玉娟,管雪强. 稳定性同位素在植物次生代谢中的研究进展[J]. 中国农学通报, 2019, 35(29): 129-133.

雷玉娟 and . The Application of Stable isotope in plant secondary metabolism: A review[J]. Chinese Agricultural Science Bulletin, 2019, 35(29): 129-133.

参考文献

[1] Kutchan T M. Ecological arsenal and developmental dis-patcher: The paradigm of secondary metabolism [J]. Plant Physiology, 2001, 125: 58-60
[2] 陈晓亚, 刘培. 植物次生代谢的分子生物学及基因工程[J]. 生命科学, 1996, 8 (2): 8-9
[3] Takafumi Shimizu, Mutsumi Watanabe, Alisdair R. Fernie, and Takayuki Tohge, Targeted LC-MS Analysis for Plant Secondary Metabolites [J]. Plant Metabolomics, 2018, 1778: 171-181
[4] 王莉, 史玲玲, 张艳霞, 刘玉军. 植物次生代谢物及其研究进展[J]. 武汉植物学研究, 2007, 25(5): 500-508
[5] Wink M. Secondary Metabolites, the Role in Plant Diversification of[J]. Encyclopedia of Evolutionary Biology, 2016: 1-9
[6] Bahmani M, Golshahi H, Saki K, Rafieian-Kopaei M, Delfan B, Mohammadie T. Medicinal plants and secondary metabolites for diabetes mellitus control [J]. Asian Pacific Journal of Tropical Disease, 2014, 4 (5): S687-S692
[7] 林光辉. 稳定同位素生态学[M]. 北京: 高等教育出版社, 2013
[8] 李国柱, 陈光. 核技术生物科学及农业应用[M]. 北京: 中国林业出版社, 2010
[9] Li SY, Gu X, Zhuang J, An TT, Pei JB, Xie HT, Li H, Fu SF, Wang JK. Distribution and storage of crop residue carbon in aggregates and its contribution to organic carbon of soil with low fertility [J]. Soil and Tillage Research, 2016, 155: 199–203
[10] Kim IY, Suh SH, Lee IK, Wolfe RR. Applications of stable nonradioactive isotope tracers in in vivo human metabolic research [J]. Experimental Molecular Medicine, 2016, 48 (12) : 20-23.
[11] An TT, Schaeffer S, Li SY, Fu SB, Pei JB, Li H, Zhuang J, Radosevich M, Wang JK. Carbon fluxes from plant to soil and dynamics of microbial immobilization under plastic mulching and fertilizer application using 13C pulse-labeling [J]. Soil Biology and Biochemistry, 2015, 80: 53–61
[12] Crone T. The basic sediment transport equations made ridiculously simple [EB/OL], ocean/ess 410, Marine Geology and Geophysics, 2014. http://www.ocean.washington. Edu /courses /ox410 /reading /sedtrans_2004.pdf
[13] Kirschman LJ, Mccue MD, Boyles JG, Warne RW. Exogenous stress hormones alter energetic and nutrient costs of development and metamorphosis [J]. Journal of Experimental Biology, 2017, 220 (18): 3391-3397
[14] Liang Y,Liu XZ,Xiao HY, Gao XF, Li WH, Xiong J Impact of high water level fluctuations on stable isotopic signature of POM and source identification in a floodplain lake-Bang Lake (Poyang Lake) [J]. Environmental Earth Sciences, 2016, 75 (3): 1-12
[15] Yoshikawa C, Abe H, Aita MN, Breider F, Kuzunuki K, Toyoda S, Ogawa NO, Suga H, Ohkouchi N, Danielache SO, Wakita M, Honda MC, Yoshida N. Insight into nitrous oxide production processes in the western North Pacific based on a marine ecosystem isotopomer model [J]. Journal of Oceanography, 2016, 72 (3): 491-508
[16] 胡桂仙, 邵圣枝, 张永志, 朱加虹, 赵首萍, 袁玉伟. 杨梅中稳定同位素和多元素特征在其产地溯源中的应用[J]. 核农学报, 2017 (12): 2450-2459
[17] Krisa S, Larronde F, Budzinski H. Stilbene Production by Vitis vinifera Cell Suspension Cultures: Methyl Jasmonate Induction and 13C Biolabeling [J]. Journal of natural products. 1999, 62 (12): 1688-1690
[18] Krisa S, Larronde F, Budzinski H. Production of 13C-labelled anthocyanins by Vitis vinifera cell suspension cultures [J]. Phytochemistry. 1999, 51(5): 651-656
[19] Muljono RAB, Scheffer JJC, Verpoorte R. Isochorismate is an intermediate in 2,3-dihy droxybenzoic acid biosynthesis in Catharanthus roseus cell cultures [J]. Plant Physiology Biochemistry, 2002, 40 (3): 231-234
[20] Steliopoulos P, Wust M, Adam KP, Mosandi A. Biosynthesis of the sesquiterpene germacrene D in Solidago Canadensis: 13C and 2H labeling studies [J]. Phytochemistry, 2002, 60 (1): 13-20
[21] Wang CZ, Maier UH, Keil M, Zenk MH, Bacher A, Rohdich F, Eisenreich W. Phenylalanine-independent biosynthesis of 1,3,5,8-tetrahydroxyxanthone [J]. European Journal of Biochemistry, 2003, 270(14): 2950-2958
[22] Poeaknapo C, Fisinger U, Zenk M, Schmidt J. Evaluation of the mass spectrometric fragmentation of codeine and morphine after 13C-isotope biosynthetic labeling [J]. Phytochemistry, 2004, 65(10): 1413-1420
[23] Takanori I, Masaki N, Kazuhiko F. Evidence for involvement of the phenylpropanoid pathway in the biosynthesis of the norligan agatharesinol [J]. Journal of plant physiology, 2006, 163(5): 483-487
[24] Yang ZY, Sakai M, Sayama H. Elucidation of the biochemical pathway of 2-phenylethanol from shikimic acid using isolated protoplasts of rose flowers [J]. Journal of Plant Physiology. 2009, 166 (8): 887-891
[25] Watanabe S, Hayashi K, Yagi K. Biogenesis of 2-phenylethanol in Rose Flowers: Incorporation of [2H8]L-Phenylalanine into 2-phenylethanol and its β-D- Glucopyranoside during the Flower Opening of Rosa “Hoh-Jun” and Rosa damascena Mill [J]. Bioscience, biotechnology, and biochemistry. 2002, 66 (5): 943-947
[26] Hayashi S, Yagi K, Ishikawa T. Emission of 2-phenylethanol from its β-D-glucopyranoside and the biogenesis of these compounds from [2H8] L-phenylalanine in rose flowers [J]. Tetrahedron. 2004, 60(3): 7005-7013
[27] 邵利,何首乌悬浮细胞系的建立和稳定同位素示踪研究二苯乙烯苷合成路径[D]. 广州: 华南理工大学,2012
[28] Hayasaka Y, Baldock GA, Pardon KH, Jeffery DW, Herderich MJ. Investigation into the formation of guaiacol conjugates in berries and leaves of grapevine Vitis vinifera L. Cv. cabernet Sauvignon using stable isotope tracers combined with HPLC-MS and MS/MS analysis [J]. Journal of Agricultural and Food Chemistry, 2010, 58 (4), 2076–2081
[29] Chassy AW, Adams DO, Laurie VF, Waterhouse AL. Tracing phenolic biosynthesis in Vitis vinifera via in situ C-13 labeling and liquid chromatographydiode-array detector-mass spectrometer/mass spectrometer detection [J]. Analytica Chimica Acta, 2012, 747, 51–57
[30] Chassy AW, Adams DO, Waterhouse AL. Tracing phenolic metabolism in Vitis vinifera berries with C-13(6)-phenylalanine: implication of an unidentified intermediate reservoir [J]. Journal of Agricultural and Food Chemistry, 2014, 62 (11), 2321–2326
[31] Chassy AW, Bueschl C, Lee H, Lerno L, Oberholster A, Barile D, Schuhmacher R, Waterhouse AL. Tracing flavonoid degradation in grapes by MS filtering with stable isotopes [J]. Food Chemistry, 2015, 166, 448–455
[32] Antoniewicz MR: Methods and advances in metabolic flux analysis: a mini-review [J]. Journal of industrial microbiology biotechnology, 2015, 42:317-325