CRISPER/Cas9系统在植物基因组定点修饰及作物遗传育种中的应用研究进展

doi:10.11924/j.issn.1000-6850.casb2021-0757

中国农学通报 ›› 2022, Vol. 38 ›› Issue (26): 9-14.doi: 10.11924/j.issn.1000-6850.casb2021-0757

所属专题：生物技术

CRISPER/Cas9系统在植物基因组定点修饰及作物遗传育种中的应用研究进展

权莹¹, 张晓娟¹(), 赵辉², 孙晓敏³, 马秀奇¹

¹陕西理工大学生物科学与工程学院,陕西汉中 723001
²陕西理工大学体育学院,陕西汉中 723001
³陕西省汉中市农业科学研究所,陕西汉中 723001

收稿日期:2021-08-11 修回日期:2021-10-22 出版日期:2022-09-15 发布日期:2022-09-09
通讯作者: 张晓娟
作者简介:权莹,女,2001年出生,陕西咸阳人,本科,研究方向为植物分子遗传。通信地址：723001 陕西省汉中市汉台区东一环路陕西理工大学生工学院。
基金资助:
陕西省科技厅项目“油菜菌核病抗性相关基因分子标记开发及种质资源创新”(2020NY-068);陕西理工大学人才启动项目“甘蓝型油菜菌核病抗性基因的筛选鉴定”(SLGRCQD2115);陕西理工大学2020年大学生创新创业训练计划项目“油菜P5CR基因克隆及其多态性与菌核病抗性相关性研究”(S202010720022)

CRISPER/Cas9 System in Plant Genome Modification and Crop Genetics and Breeding: Research Progress

QUAN Ying¹, ZHANG Xiaojuan¹(), ZHAO Hui², SUN Xiaomin³, MA Xiuqi¹

¹School of Biological Sciences and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723001
²Department of Physical Education, Shaanxi University of Technology, Hanzhong, Shaanxi 723001
³Hanzhong Agricultural Science Institute, Hanzhong, Shaanxi 723001

Received:2021-08-11 Revised:2021-10-22 Online:2022-09-15 Published:2022-09-09
Contact: ZHANG Xiaojuan

摘要/Abstract

摘要：

CRISPR/Cas9系统是一项简单、高效的基因定点编辑技术,在植物遗传改良及作物良种选育等方面具有重要的应用价值。本研究主要介绍了CRISPR/Cas9的原理及构建方法,论述了近年来CRISPR/Cas9技术在植物基因功能及基因表达调控、植物基因组的定向编辑以及作物分子育种中的应用及研究进展,分析了该基因编辑系统的主要影响因素及优化改进方式,探讨了该系统在应用中的问题及解决途径并对今后发展方向进行了展望,为该技术在植物基因组定点编辑及作物遗传育种等领域研究提供参考。

关键词: CRISPER/Cas9, 基因编辑, CRISPR/Cas9系统影响因素, 分子设计育种, 作物遗传育种

Abstract:

The CRISPR/Cas9 system is a simple and efficient targeted gene editing technology, which has important application value in plant genetic improvement and crop genetics and breeding. This research mainly introduced the principle and construction method of CRISPR/Cas9, discussed the application and research progress of CRISPR/Cas9 in plant gene function and gene expression regulation, plant genome site-specific editing and crop molecular breeding in recent years. We analyzed the main influencing factors and the optimizing methods of this gene editing technology, as well as problems in its application and the solutions to the problems, and discussed the future development direction of the technology, thus providing reference for the application of CRISPR/Cas9 in plant genome editing and crop genetics and breeding.

Key words: CRISPER/Cas9, gene editing, influencing factors of CRISPR/Cas9 system, molecular design breeding, crop genetics and breeding

中图分类号:

权莹, 张晓娟, 赵辉, 孙晓敏, 马秀奇. CRISPER/Cas9系统在植物基因组定点修饰及作物遗传育种中的应用研究进展[J]. 中国农学通报, 2022, 38(26): 9-14.

QUAN Ying, ZHANG Xiaojuan, ZHAO Hui, SUN Xiaomin, MA Xiuqi. CRISPER/Cas9 System in Plant Genome Modification and Crop Genetics and Breeding: Research Progress[J]. Chinese Agricultural Science Bulletin, 2022, 38(26): 9-14.

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CRISPER/Cas9系统在植物基因组定点修饰及作物遗传育种中的应用研究进展

CRISPER/Cas9 System in Plant Genome Modification and Crop Genetics and Breeding: Research Progress

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