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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (25): 127-131.doi: 10.11924/j.issn.1000-6850.casb2023-0698

• 食品·营养·检测·安全 • 上一篇    下一篇

云南马关县红果参果实花色苷的紫外-可见光谱和含量

顾怀珊1(), 钟正阳2, 杨迪1, 赵凯玲2, 张万巧2, 邓国军2, 黄兴粉2, 王献2, 赵强彪2, 陆玲2(), 李仕江1, 赵昶灵1(), 杨忠益3   

  1. 1 云南农业大学农学与生物技术学院，昆明 650201
    2 文山壮族苗族自治州农业科学院，云南文山 663000
    3 文山壮族苗族自治州马关县马关忠益种植园，云南马关 663700
  • 收稿日期:2023-09-27 修回日期:2024-04-03 出版日期:2024-09-05 发布日期:2024-08-27
  • 通讯作者:
    陆玲，女，1975年出生，云南广南人，高级农艺师，硕士，主要从事果树育种研究。通信地址：663000 云南省文山壮族苗族自治州文山市泰康西路2号 文山壮族苗族自治州农业科学院园艺研究所，Tel：0876-3037529，E-mail：。
    赵昶灵，男，1969年出生，四川都江堰人，教授，博士，主要从事药用植物产量和品质形成及其调控机理的研究。通信地址：650201 云南省昆明市盘龙区沣源路452号 云南农业大学农学与生物技术学院，Tel：0871-65227213，E-mail：。
  • 作者简介:

    顾怀珊，女，2000年出生，云南昆明人，在读硕士，主要从事药用植物产量和品质形成及其调控机理的研究。通信地址：650201 云南省昆明市盘龙区沣源路452号 云南农业大学农学与生物技术学院，Tel：0871-65227213，E-mail：。

  • 基金资助:
    文山壮族苗族自治州农业科学院资助项目“滇东南石漠化地区特色饲草种质资源评价和优良种质筛选”(WYHZ2023-01); 文山壮族苗族自治州财政局资助项目“文山州特色水果创新育种与绿色高效栽培技术集成研究”(46)

Ultraviolet-Visible Spectra and Contents of Fruit Anthocyanins of Cyclocodon lancifolius in Maguan County of Yunnan Province

GU Huaishan1(), ZHONG Zhengyang2, YANG Di1, ZHAO Kailing2, ZHANG Wanqiao2, DENG Guojun2, HUANG Xingfen2, WANG Xian2, ZHAO Qiangbiao2, LU Ling2(), LI Shijiang1, ZHAO Changling1(), YANG Zhongyi3   

  1. 1 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201
    2 Academy of Agricultural Sciences of Wenshan Zhuang and Miao Autonomous Prefecture, Wenshan, Yunnan 663000
    3 Zhongyi Plantation of Maguan County, Maguan, Yunnan 663700
  • Received:2023-09-27 Revised:2024-04-03 Published:2024-09-05 Online:2024-08-27

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摘要：

研究旨在阐明云南省马关县红果参品系果实花色苷的紫外-可见光谱和含量，为马关县乃至云南省的红果参品种选育以及果实的开发、利用和营销提供依据。以马关县红果参品系‘文黑果1号’~‘文黑果8号’为材料，基于在200~700 nm范围内的连续扫描检测了果实花色苷的紫外-可见光谱，用分光光度法测定了果皮、果肉和果实的总花色苷含量(TAC)。结果表明，8个红果参品系果实花色苷1%浓盐酸-甲醇提取液的紫外-可见光谱中，在可见光区和紫外光区分别存在1个特征性吸收带，在可见光区的最大吸收峰波长为537.0 nm。果皮、果实TAC均最高的是‘文黑果2号’，分别约为(3.546±0.424)、(1.758±0.070) A537.0/(g·FW)；TAC果皮/TAC果实最高的是‘文黑果5号’，约为2.237±0.096；TAC果皮/TAC果肉最高的是‘文黑果6号’，约为14.911±1.729；‘文黑果7号’的果肉TAC、TAC果肉/TAC果实均最高，分别约为0.809±0.010 A537.0/(g·FW)、1.284±0.056。此外，所有TAC相关指标在8个品系间的差异均达到极显著水平。

关键词: 红果参, 果实花色苷, 紫外-可见光谱, 云南省马关县

Abstract:

This research aimed to clarify the ultraviolet-visible (UV-vis) spectra and contents of the fruit anthocyanins of the Cyclocodon lancifolius strains in Maguan County, Yunnan Province, providing a basis for the breeding, development, utilization and marketing of the fruits of the C. lancifolius in the county, and even in Yunnan Province. The C. lancifolius strains, i.e., ‘Wenheiguo No.1’- ‘Wenheiguo No.8’, in the county were employed as the test materials. The UV-vis spectra of the fruit anthocyanins were determined based on the continuous scanning in the range of 200-700 nm, and the total anthocyanin contents (TACs) of the fruits including the peels and flesh were evaluated by using spectrophotometry. The results showed that, in the UV-vis spectra of 1% concentrated hydrochloric acid-methanol extracts of the fruit anthocyanins of the eight C. lancifolius strains, one specific absorption band emerged in the visible and ultraviolet light regions, respectively, and the maximum absorption peak wavelength in the visible light region was 537.0 nm. ‘Wenheiguo No.2’ had the highest TACs for both the peels and the whole fruits, i.e., about (3.546±0.424) A537.0/(g·FW) and (1.758±0.070) A537.0/(g·FW), respectively, ‘Wenheiguo No.5’ held the highest TAC ratio of the peels to the whole fruits, i.e., about 2.237±0.096, ‘Wenheiguo No.6’ possessed the highest TAC ratio of the peels to the flesh, i.e., about 14.911±1.729, and the flesh TAC and the TAC ratio of the flesh to the whole fruits of ‘Wenheiguo No.7’ were both the highest, i.e., about (0.809±0.010) A537.0/(g·FW) and 1.284±0.056, respectively. In addition, the differences of all TAC-related indexes among the eight strains reached extremely significant.

Key words: Cyclocodon lancifolius, fruit anthocyanin, ultraviolet-visible spectrum, Maguan County, Yunnan Province