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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (17): 142-148.doi: 10.11924/j.issn.1000-6850.casb2023-0865

所属专题： 生物技术； 畜牧兽医

• 畜牧·动物医学·蚕·蜂 • 上一篇    下一篇

牛体外胚胎冷冻在生产中的应用效果

孙伟1,2(), 赵勇超1, 张浩波1, 王超1, 郭雅欣1, 王乐群1, 王飞飞1, 刘涛1, 张博钊1, 乔嘉明1, 李喜和1,3()   

  1. 1 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司，呼和浩特 011517
    2 内蒙古国家乳业技术创新中心有限责任公司，呼和浩特 010020
    3 内蒙古大学生命科学学院蒙古高原动物遗传资源研究中心，呼和浩特 010070
  • 收稿日期:2023-12-08 修回日期:2024-03-31 出版日期:2024-06-15 发布日期:2024-06-11
  • 通讯作者:
    李喜和，男，1963年出生，内蒙古呼和浩特人，教授，博士，研究方向：动物生殖生物学与生物技术。通信地址：011517 内蒙古和林格尔县盛乐经济园区与云谷大道东段交汇处 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司，Tel：0471-2383200，E-mail：。
  • 作者简介:

    孙伟，男，1979年出生，内蒙古乌兰察布人，高级畜牧师，博士，研究方向：动物遗传育种与繁殖。通信地址：011517 内蒙古和林格尔县盛乐经济园区与云谷大道东段交汇处 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司，Tel：0471-2383200，E-mail：。

  • 基金资助:
    创建国家乳业技术创新中心重点专项“低碳排放与抗热应激奶牛培育关键技术研究及奶羊引种扩繁技术创新”(2022-科研攻关-3); 内蒙古自治区科技计划项目“奶牛良种选育关键技术开发”(2021GG0403)

The Application Effect of Bovine in vitro Embryo Freezing in Production

SUN Wei1,2(), ZHAO Yongchao1, ZHANG Haobo1, WANG Chao1, GUO Yaxin1, WANG Lequn1, WANG Feifei1, LIU Tao1, ZHANG Bozhao1, QIAO Jiaming1, LI Xihe1,3()   

  1. 1 Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot 011517
    2 Inner Mongolia National Dairy Technology Innovation Center Co., Ltd, Hohhot 010020
    3 Institute for Animal Genetic Resources of Mongolia Plateau, College of Life Science, Inner Mongolia University, Hohhot 010070
  • Received:2023-12-08 Revised:2024-03-31 Published:2024-06-15 Online:2024-06-11

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摘要：

本研究旨在评估不同牛体外胚胎冷冻保存方案的效率，以优化技术流程。研究比较了仪器玻璃化与手工玻璃化冷冻牛体外胚胎的2 h复苏率、24 h孵化率及45 d初检妊娠率；比较2种程序化冷冻方案冷冻牛体外胚胎的2 h复苏率、24 h孵化率及48 h孵化率；对比牛体外鲜胚、体外冻胚以及体内冻胚45 d移植妊娠率。仪器玻璃化冷冻方案与程序化冷冻方案解冻胚胎的2 h复苏率(72.91%±5.52% vs. 70.91%±6.75%)、24 h孵化率(60.42%±6.33% vs. 58.18%±5.62%)均不具有显著性差异(P>0.05)。程序化冷冻中过蔗糖方案的冷冻-解冻胚胎的24 h孵化率(44.23%±3.33% vs. 37.50%±3.58%)显著高于不经过蔗糖的冷冻方案(P<0.05)。牛体外鲜胚、体外冻胚以及体内冻胚移植45 d初检妊娠率分别为56.81%±6.27%、45.00%±6.13%、49.41%±7.55%。在实际应用中，牛体外生产胚胎的冷冻效果与体内胚胎相比仍有一定的差距。综上所述，在牛体外胚胎的冷冻保存中，仪器玻璃化与手工玻璃化的效果相当，而程序化冷冻方案中使用高浓度蔗糖可以显著提升冷冻效率。

关键词: 牛体外胚胎, 玻璃化, 程序化, 妊娠率, 冷冻保存

Abstract:

The aim is to screen for more efficient cryopreservation schemes for bovine in vitro embryos by comparing the effects of different vitrification and programmed cryopreservation schemes. The 2-hour recovery rate, 24-hour hatching rate, and 45 day initial pregnancy rate between instrument vitrification and manual vitrification frozen bovine embryos in vitro were compared; the 2-hour recovery rate, 24-hour hatching rate, and 48-hour hatching rate of frozen bovine in vitro embryos between two programmed freezing schemes were compared; and the pregnancy rates of 45 day transplantation of fresh embryos in vitro, frozen embryos in vitro, and frozen embryos in vitro in cattle were compared. There was no significant difference in the 2-hour recovery rate (72.91%±5.52% vs.70.91%±6.75%) and 24-hour hatching rate (60.42%±6.33% vs. 58.18%±5.62%) between the instrument vitrification freezing scheme and the programmed freezing scheme for thawed embryos (P>0.05). The 24-hour hatching rate of frozen thawed embryos with sucrose scheme in programmed freezing was significantly higher than that of the freezing scheme without sucrose (44.23%±3.33% vs. 37.50%±3.58%) (P<0.05). The 45 days examination pregnancy rates of fresh bovine embryos in vitro, frozen bovine embryos in vitro, and frozen bovine embryos in vivo were 56.81%±6.27%, 45.00%±6.13%, and 49.41%±7.55%, respectively. In practical applications, there is still a certain gap in the freezing effect of bovine embryos produced in vitro compared to in vivo embryos. There is no significant difference in the effect between instrument vitrification freezing and manual vitrification freezing. The use of sucrose in the programmed freezing scheme can significantly improve the freezing effect.

Key words: in vitro embryo of cattle, vitrification, programmatization, pregnancy rate, cryopreservation