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中国农学通报 ›› 2010, Vol. 26 ›› Issue (21): 90-93.

• 林学 园艺 园林 • 上一篇    下一篇

巨大芽胞杆菌在3种固沙植物根部的定殖

王天龙 李永 郭民伟 张晓娟 汪来发 朴春根   

  • 收稿日期:2010-05-17 修回日期:2010-08-29 出版日期:2010-11-05 发布日期:2010-11-05
  • 基金资助:

    国家科技基础条件平台建设项目

Bacillus megaterium Colonization in Roots on Three Sand-fixing Plants

  • Received:2010-05-17 Revised:2010-08-29 Online:2010-11-05 Published:2010-11-05

摘要:

用200μg?mL-1的卡那霉素标记巨大芽胞杆菌菌株R,并获得抗药标记菌株,明确其在固沙植物杨柴、柠条和油蒿根上的定殖能力。将标记菌株制成菌液,浸种接种至杨柴、柠条和油蒿的种子,待种苗根系生长至约12cm后,测定R菌株在三种植物不同根段的定殖密度和根际效应。结果表明标记菌株在灭菌土壤中定殖密度比在自然土中大,根系不同部位标记菌株定殖密度表现为从上到下逐渐减小,0~3cm根段标记菌株在根际的定殖密度达到最大,9~12cm根段几乎不能分离到标记菌株。三种植物中,杨柴的根际效应最显著,标记菌株在油蒿根际的定殖密度最大。

关键词: 有机高聚物包膜肥, 有机高聚物包膜肥, 养分释放速率

Abstract:

Bacillus megaterium strain R labeled by kanamycin was obtained after being marked with concentration 200μg?mL-1 kanamycin and the seeds of Hedysarum mongolicum, Caragana korshinskii and Artemisia ordosica were inoculated with the strain R solution by soaking it to evaluate the colonization density of R strain and rhizosphere effect of different root parts of these plants after the seeding root tip to 12 cm. The result showed that the density of R strain in sterilized soil was more than it in natural soil. The colonization density of strain R came from the root tip down gradually in different root parts, and the colonization density reached to maximum in the 0~3 cm part. The strain R could not be isolated in the 9~12 cm part. The rhizosphere effect of H. mongolicum was most prominent in these plants, and the colonization density of strain R reached to maximum in the rhizosphere of A. ordosica.