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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (7): 300-303.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

脂质体转染陆川猪胎儿成纤维细胞条件的优化

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  • 收稿日期:2010-10-09 修回日期:2010-12-22 出版日期:2011-04-05 发布日期:2011-04-05

Optimization of Parameters of Exogene Transfection of Luchuan Pig Fetal Fibroblasts in vitro Mediated by Liposome

  • Received:2010-10-09 Revised:2010-12-22 Online:2011-04-05 Published:2011-04-05

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摘要：

本研究旨在脂质体介导基因转染陆川猪胎儿成纤维细胞时获得较高转染效率，同时以GFP为报告基因探讨影响外缘基因转染效率的参数，如DNA和脂质体的用量、转染的细胞数量及细胞与DNA与脂质体复合物的孵育时间。结果表明, 6μgDNA与15μl脂质体转染8h，可获得较满意的转染效率.

关键词: 云南重楼 有机肥 无机肥 产量, 云南重楼 有机肥 无机肥 产量

Abstract:

This study was aimed to obtain higher efficiency in gene transfection into Luchuan Pig Fetal Fibroblasts and to study the role of green fluorescence proten (GFP) as a reporter system. We investigated the effect of parameter such as the dose of DNA and liposome number of cell transfected and exposure time of the cell to the DNA-liposome complexes. It was indicated that 6ug DNA with 15uL liposome resulted in the highest transfection efficiency after 8h transfection