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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (27): 270-276.

• 食品 营养 检测 安全 • 上一篇    下一篇

富硒与富锗桦褐孔菌多酚的体内外抗氧化作用

蔡建秀 黄晓冬 吴少红 郑绪兰   

  • 收稿日期:2011-11-22 修回日期:2012-02-13 出版日期:2012-09-25 发布日期:2012-09-25
  • 基金资助:

    桦褐孔菌深层培养及功能研究与开发;泉州师范学院福建省高校服务海西建设重点项目;泉州师院开放实验室项目;泉州师院大学生创新实验项目

In Vitro and in Vivo Antioxidant Effects and Mechanism of Selenium –enriched and Germanium –enriched Inonotus obliquus polyphenol

cai jianxiu   

  • Received:2011-11-22 Revised:2012-02-13 Online:2012-09-25 Published:2012-09-25

PDF (PC)

655

摘要：

研究桦褐孔菌多酚(IOP)以及富硒培养的桦褐孔菌多酚(Se-IOP)、富锗培养的桦褐孔菌多酚(Ge-IOP)的抗氧化作用。以试剂盒法测定抗活性氧单位、MDA值，分光光度法测定脂肪氧合酶活力、红细胞溶血抑制率、谷胱甘肽过氧化物酶活力。在化学模拟体系中，4.0 mg/mL的IOP、Se-IOP与Ge-IOP的抗氧化活性单位分别为52.36、89.21、82.93 U/mL，对脂肪氧合酶活性的抑制率分别为34.15%、42.22%、50.53%。在细胞与组织水平的体外抗氧化体系中，4.0 mg/mL IOP对H2O2诱导的小鼠红细胞溶血抑制率为69.65%，而1~4.0 mg/mL Se-IOP与Ge-IOP溶血抑制率均大于100%；与对照组比较，1~4.0 mg/mL IOP、Se-IOP与Ge-IOP均可极显著地抑制小鼠肝匀浆自发性产生MDA(P<0.01)。个体水平的体内抗氧化体系中，腹腔注射IOP、 Se-IOP与Ge-IOP(4.0 mg/mL，0.5 mL/d，12天)时，肝组织的自发性MDA指标均极显著下降(P<0.01)；腹腔注射1~4.0 mg/mL Se-IOP与Ge-IOP后，各剂量组小鼠血清与肝匀浆谷胱甘肽过氧化物酶GSH－PX活力均极显著增强(P<0.01)。IOP、Se-IOP与Ge-IOP在体内外均具有明显的抗氧化作用，富硒、富锗可增强桦褐孔菌多酚的抗氧化能力。

关键词: 利用途径, 利用途径

Abstract:

The aim was to investigate the antioxidant effects of Inonotus obliquus polyphenol (IOP), Selenium-enriched Inonotus obliquus polyphenol (Se-IOP) and Germanium-enriched Inonotus obliquus polyphenol (Ge-IOP). The reagent kit methods were used to determine anti-reactive oxygen species (anti-ROS) unit and malondialdehyde (MDA) value, the spectrophotometry methods were used to determine lipoxygenase activity, inhibitory rate of red blood cell (RBC) hemolysis and glutathione peroxidase (GSH-Px) activity, respectively. In chemical modified systems, the antioxidant activity of 4.00 mg/mL IOP, Se-IOP and Ge-IOP were 52.36, 89.21 and 82.93 anti-ROS units, the inhibiting rates on lipoxygenase were 34.15%, 42.22% and 50.53%, respectively. In vitro cellular and tissular antioxidant systems, the inhibitory rate of 4.00 mg/mL IOP on mice RBC hemolysis by H2O2 inducement was 69.65%, which of 1-4.0 mg/mL Se-IOP and Ge-IOP were above 100%. The inhibitory effects of 1-4.0 mg/mL IOP, Se-IOP and Ge-IOP on the formation of MDA in mice without inducement were very significantly as compared with control group (P<0.01). In vivo antioxiddant systems, the MDA value in mice liver decreased very significantly when the mice were intra-peritoneal injected with IOP, Se-IOP and Ge-IOP (4.0 mg/mL,0.5 mL/d, 12 d) respectively(P<0.01), the GSH-Px activities in mice serum and liver also increased very significantly after being injected with Se-IOP and Ge-IOP(1-4.0 mg/mL,0.5 mL/d, 12 d) respectively (P<0.01). IOP, Se-IOP and Ge-IOP had antioxidant effect in vitro and in vivo, Selenium and Germanium could increase the antioxidant activity of Inonotus obliquus polyphenol.