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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (27): 45-48.

所属专题： 生物技术； 油料作物

• 农学 农业基础科学 • 上一篇    下一篇

不同基因型对花生胚小叶植株再生的影响

隋炯明 乔利仙 赵明霞 徐丽娟 王晶珊   

  • 收稿日期:2012-04-26 修回日期:2012-06-18 出版日期:2012-09-25 发布日期:2012-09-25
  • 基金资助:

    花生几丁质酶基因、β-1,3-葡聚糖酶基因启动子的克隆与功能分析;花生维生素E合成关键基因的克隆及其抗氧化机制研究;花生Chi基因及Glu基因的克隆、双价表达载体的构建及遗传转化研究

Effect of Genotypes on Plant Regeneration of Embryonic Leaflet in Peanut

  • Received:2012-04-26 Revised:2012-06-18 Online:2012-09-25 Published:2012-09-25

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摘要：

以不同类型18个花生品种成熟种子的胚小叶为外植体，对不定芽诱导及植株再生进行了研究，旨在为花生遗传转化和离体诱变等提供培养方法。将胚小叶外植体培养在添加1 mg/L NAA和6 mg/L BAP的诱导培养基上，4周后转移到添加4 mg/L BAP的培养基上进行培养。结果表明，所有的供试品种芽点诱导率均高于60%，但5大类型间及品种间(62.2%~95.5%)存在显著差异，龙生型平均诱导率最高(87.2%)，其次是珍珠豆型(81.5%)，多粒型最低(63.1%)。将形成芽点的外植体转移到添加4 mg/L BAP的培养基上后，部分外植体从芽点上分化出不定芽，继续培养不定芽伸长并再生植株。植株再生率也存在显著性差异，最高的是珍珠豆型(83.5%~97.1%)，最低是多粒型(14.8%~22.0%)。

关键词: 淋溶模拟, 淋溶模拟

Abstract:

To provide culture method for genetic transformation and tissue culture in vitro, efficient adventitious shoot induction and plant regeneration of embryonic leaflets among 18 peanut cultivars in 5 types were studied in this paper. The embryonic leaflets were cultured on MSB5 induction medium (Murashige and Skoog salts plus B5 vitamins) supplemented with 1 mg/L NAA and 6 mg/L BAP, and then transferred to the medium supplemented with 4 mg/L BAP. The result showed that the frequencies of bud point induction were different significantly in the cultivars tested that ranged 62.2%-95.5% as well as in 5 peanut types. The highest induction frequency was obtained in Arachis hypogaea var. hirsuta (87.2%), followed by Arachis hypogaea var. rulgaris (81.5%), the lowest was Arachis hypogaea var. fastigiata (63.1%). When the explants with bud points were transferred to the medium supplemented with 4 mg/L BAP, some of the bud points developed and then regenerated plants. The regeneration rate of plant was also different, the cultivars in Arachis hypogaea var. rulgaris produced higher frequencies (83.5%-97.1%), while that of Arachis hypogaea var. fastigiata produced lower frequencies (14.8%-22.0%).