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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (13): 25-30.

所属专题： 生物技术； 农业生态

• 林学 园艺 园林 • 上一篇    下一篇

VA菌根对黄檗幼苗抗性生理指标的影响

李桂伶 范继红   

  • 收稿日期:2010-11-16 修回日期:2011-01-06 出版日期:2011-06-05 发布日期:2011-06-05

VA Mycorrhizal Influence on the Phellodendron amurense Seedling Resistance of Physiological Indexes

  • Received:2010-11-16 Revised:2011-01-06 Online:2011-06-05 Published:2011-06-05

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摘要：

通过盆栽接种试验，研究VA菌根对黄檗（Phellodendron amurense）1年生实生苗的抗性生理指标的影响。结果表明，VA菌根可明显提高黄檗幼苗的抗性指标。可溶性糖含量随着侵染率的增加而增大，并能促进黄檗叶片中可溶性糖向根系中转移，游离脯氨酸和MDA含量逐渐降低，呼吸酶活性和保护酶活性增强。接种Glomus diaphanum的苗木可溶性糖，游离脯氨酸、CAT活性及POD活性变化最为明显，叶片可溶性糖含量为0.5736%，叶片脯氨酸含量为16.27 μg/g，叶片CAT活性为0.3186 mg/(g?min)，叶片POD活性为258.32 u/(g?min)。接种G. mosseae对黄檗苗木MDA含量、呼吸酶活性及SOD活性影响最大，叶片MDA含量为0.0119 μmol/g，叶片抗坏血酸氧化酶活性为0.2714 mg/(g?min)，叶片多酚氧化酶活性为0.3487 mg/(g?min)，叶片SOD活性为274.18 u/(g?FW)。

关键词: 对策研究, 对策研究

Abstract:

According to the potted inoculation experiment, we studied on VA mycorrhizalhad influenced on Phellodendron amurense resistance physiological indexes of annual seedlings. The results showed that VA mycorrhizal fungi significantly increased the resistance physiological index of Phellodendron amurense seedlings, the content of soluble sugar increased by the enlarged infection rates; meanwhile promoted the soluble sugar in the leaves of Phellodendron amurense transferring to the roots, gradually decreased the content of Fpro and MDA and increased activities of respiratory enzyme and protective enzymes. After inoculated Glomus diaphanum, the seedling showed that soluble sugar, Fpro, the activity of CAT and POD had the most obviously changes. The content of soluble sugar of the leaf was 0.5736%, the content of leaf’s Fpro was 16.27μg/g, the activity of leaf CAT was 0.3186 mg/(g?min)，the activity of leaf‘s POD was 258.32 u/(g?min). It had the greatest infect on the MDA content, the activity respiratory enzyme and SOD after inoculated G. mosseae for Phellodendron amurense seedlings. The content of MDA of the leaf was 0.0119 μmol/g, the activity leaf ascorbic acid oxidase was 0.2714 mg/(g?min), the activity of leaf’s polyphenol oxidase was 0.3487 mg/(g?min), the activity of leaf SOD was 274.18 u/(g?FW).