关键词: 免疫应答, 免疫应答, 中性粒细胞, 抗菌肽

Abstract:

Mytilus coruscus widely spreads all through the littoral extent of East China Sea with important economic value. Abundant peptides with antimicrobial activity were identified from hemocytes of mussel in previously works. To elucidate the compositive characterization of antimicrobial peptides from blood cell and serum of Mytilus coruscus hemocytes, high performance liquid chromatogram were used and several fractions with distinct antimicrobial activity were isolated from blood cell and serum of Mytilus coruscus, respectively. Mass spectrometry combined cDNA data analysis were performed to characterize the molecular mass and annotation of some peptides. Some of antimicrobial peptides identified in this work were considered belong to Mytilins or Myticins family with molecular mass ranged from 3000 to 7000 Da. The difference between blood cell and serum antimicrobial peptides mainly exists in molecular composition and antimicrobial spectrum. In addition, a novel peptide with strong antimicrobial activity was found in serum of Mytilus coruscus and the molecular mass of this peptide is more than 10000 Da. The results of this work build the foundation for further research on the molecular diversity of antimicrobial peptides, immune mechanisms of Mytilus coruscus, and screening for new peptides with applicable value.