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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 220-226.

• 林学 园艺 园林 • 上一篇    下一篇

多菌灵降解菌XJ-D的分离鉴定及特性研究

林秀 侯振安 赵思峰 谢磊 李会杰 李谟志   

  • 收稿日期:2010-12-23 修回日期:2011-01-11 出版日期:2011-07-05 发布日期:2011-07-05
  • 基金资助:

    国家科技支撑计划课题

Isolation and Characteristics of Carbendazim Degradation Bacterium XJ-D

  • Received:2010-12-23 Revised:2011-01-11 Online:2011-07-05 Published:2011-07-05

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摘要：

为了进行多菌灵污染土壤的生物修复，笔者从长期施用多菌灵农药的葡萄园中分离筛选得到1株多菌灵降解菌XJ-D，经Biolog微生物自动分析系统和16S rDNA序列比对，以及系统聚类分析等鉴定菌株XJ-D为红串红球菌（Rhodococcus erythropolis）。多菌灵降解菌XJ-D可利用多菌灵为唯一碳源、氮源进行生长，将其接种在600 mg/L多菌灵的无机盐培养基中，11天时多菌灵的降解率达99.0%，平均降解能力为52.87 mg/(L?d)。

关键词: 宜兴市, 宜兴市

Abstract:

In order to take bioremediation of carbendazim contaminated soil, an bacterial strain XJ-D, capable of degrading carbendazim was isolated by continuous enrichment culture from grape vineyard soil, shihezi in xinjiang. Identification of bacterial strain was completed with Biolog analysis and comparison sequences of 16S rDNA and phylogenetic analysis. A bacterial strain of XJ-D was identified as Rhodococcus erythropolis. It could use carbendazim as sole carbon or nitrogen source. XJ-D can degrade 99.0% of carbendazim at concentrations of 600 mg/L in mineral salts medium within 11 days. It showed a high average degradation rate of 52.87 mg/(L?d) for XJ-D.