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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 215-219.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

菠萝蜜的染色体制片优化及核型分析

劳世辉 魏岳荣 盛鸥 梁国鲁   

  • 收稿日期:2011-03-24 修回日期:2011-05-20 出版日期:2011-07-05 发布日期:2011-07-05
  • 基金资助:

    国家科技支撑项目;科技部国际合作项目

Optimization of Chromosome Mounting Technique and Karyotype Analysis of Jackfruit (Artocarpus heterophyllus Lam.)

  • Received:2011-03-24 Revised:2011-05-20 Online:2011-07-05 Published:2011-07-05

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摘要：

应用改良的去壁低渗法研究比较了不同预处理剂对菠萝蜜染色体制片的效果及其染色体数目和核型。结果表明：以0.002 mol/L 8-羟基喹啉与0.2%秋水仙素（1:1）混合液作为预处理剂，预处理3 h效果较好。菠萝蜜的染色体数目为2n=2x=56，为二倍体，主要由中部和近中部着丝点染色体构成，9号染色体上附着一对随体；最长染色体/最短染色体为2.08:1，臂比大于2的染色体占全组染色体的3.5%，属2A核型，核型公式为2n=2M+50m（2SAT）+4sm。该文首次发现菠萝蜜可能存在B染色体。

关键词: 遗传多样性, 遗传多样性

Abstract:

In this paper, the chromosome number and karyotype of jackfruit was studied after detaching the cell wall and treated with low osmotic solution, and the effects of different pretreatments were also investigated. The results showed that the optimum pretreatment chemical was the mixture of 0.2% colchicine and 0.002 mol/L 8-Hydroxyquinoline (1:1), with the pretreatment time for 3 hours. The karyotype analysis showed that jackfruit was diploid (2n=2x=56). The ratio of the longest chromosome to the shortest one is 2.08:1, and the arm ration of 3.5% chromosomes is over 2, so that the karyotype formula of jackfruit is 2n=2M+50m (2SAT) +4sm, belonging to 2A karyotype. It is the first report that the B Chromosome may be in jackfruit.