‘赞皇大枣’增殖培养研究

中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 210-214.

‘赞皇大枣’增殖培养研究

王艳彭建营

收稿日期:2011-01-04 修回日期:2011-02-24 出版日期:2011-07-05 发布日期:2011-07-05
基金资助:
中国枣属植物分子系统学及枣的品种分类学;与枣无核性状相关联基因的差异表达研究;苹果花孕育相关基因的鉴别、克隆和表达研究

Studies on Proliferation of Ziziphus jujuba ‘Zanhuang Dazao’

Received:2011-01-04 Revised:2011-02-24 Online:2011-07-05 Published:2011-07-05

摘要/Abstract

摘要：

以‘赞皇大枣’组培苗为材料，研究6-BA、IBA和NAA在枣组培苗增殖培养中的效应并筛选出适宜组合。结果表明，在赞皇大枣组培苗增殖培养中，6-BA分别与IBA和NAA配合使用时，IBA的活性高于NAA，二者结合使用可显著促进组培苗的增殖并提高成苗质量。‘赞皇大枣’最适培养基为MS+6-BA 1.5 mg/L+IBA 0.1 mg/L，最适培养条件为温度(25±2)℃，光照强度2000~3000 lx，光照时间14 h/d。

关键词: 保存技术, 保存技术

Abstract:

Use tissue culture seedlings of Ziziphus jujuba Mill. ‘Zanhuangdazao’ as Use tissue culture seedlings of Ziziphus jujuba ‘Zanhuang Dazao’ as explants, effects of 6-BA, IBA and IAA on proliferation of Ziziphus jujuba were studied and selected its suitable composition in subculture. The results showed that in subculture, activity of IBA was higher, as compare with NAA. 6-BA blended with IBA can remarkably promote multiplication coefficient and quality of grow into seedlings. The best medium of Ziziphus jujuba ‘Zanhuang Dazao’ was MS+6-BA 1.5 mg/L+IBA 0.1 mg/L. The best culture conditions for the temperature (25±2)℃, light intensity 2000~3000 lx, illumination time 14 h/d.

中图分类号:

S665.1

王艳彭建营. ‘赞皇大枣’增殖培养研究[J]. 中国农学通报, 2011, 27(16): 210-214.