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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 206-210.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

苹果茎尖组培苗继代保存多年后繁殖特性研究

刘颖 师校欣 杜国强 常腾飞   

  • 收稿日期:2010-12-13 修回日期:2011-02-15 出版日期:2011-07-05 发布日期:2011-07-05
  • 基金资助:

    不同继代次数苹果组培苗种质保存特性研究

Organogenesis Characteristics of Apple Shoot Explants in vitro after Subcultured for Years

  • Received:2010-12-13 Revised:2011-02-15 Online:2011-07-05 Published:2011-07-05

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摘要：

为解决种质离体保存的适宜年限等实用性问题，需了解多年继代保存的组培苗繁殖能力有无变化。本试验对继代保存不同年限的 ‘金冠’、‘乔纳金’和‘嘎拉’、‘富士’茎尖组培苗进行继代增殖能力、不定根诱导和离体叶片不定芽再生能力的研究。结果表明：外植体接种初期4年以内，即继代次数为24或40代时，‘富士’、‘金冠’、‘乔纳金’的新梢增殖能力较低，‘嘠拉’生根率较低，‘金冠’、‘富士’、‘嘠拉’离体叶片再生率较低，随着培养时间延长，继代次数增加，以上品种的继代增殖效率、生根能力及离体叶片再生能力均稳定在较高水平。长期继代保存的苹果品种茎尖组培苗（‘富士’已保存25年，‘金冠’、‘乔纳金’19年，‘嘠拉’10年）仍保持其器官再生能力。

关键词: RNA提取, RNA提取

Abstract:

For figure out the optimal life span of germplasm preserved in vitro, it is necessary to understand whether its propagating ability changes or not after subcultured for years. The shoot multiplication ability, adventitious root regeneration rate from shoots, and adventitious bud regeneration rate from leaves of ‘Fuji’, ‘Golden Delicious’, ‘Jonagold’ and ‘Gala’ apple in vitro shoot explants were studied. The results showed that it was lower that the shoot multiplication ability of ‘Fuji’, ‘Golden Delicious’ and ‘Jonagold’, the adventitious root regeneration rate of ‘Gala’, and the adventitious bud regeneration rate from leaves of ‘Golden Delicious’ , ‘Fuji’, and ‘Gala’ during the first four years after inoculation from field, that was at the stage of subculture from 24 to 40 times. With the increasing of the subculture times, the abilities for the given cultivars could increase and keep at a higher level stably. The apple explants repeatedly subcultured in vitro for years (25 years for ‘Fuji’, 19 years for ‘Golden Delicious’ and ‘Jonagold’, and 10 years for ‘Gala’) could remain their organogenesis characteristics.