欢迎访问《中国农学通报》,

中国农学通报 ›› 2021, Vol. 37 ›› Issue (21): 134-139.doi: 10.11924/j.issn.1000-6850.casb2020-0765

所属专题: 生物技术

• 生物科学 • 上一篇    下一篇

基于ISSR标记的5个蓼蓝居群遗传多样性分析

徐明(), 汪琼, 李冬玲, 郑玉红, 徐增莱()   

  1. 江苏省中国科学院植物研究所,南京 210014
  • 收稿日期:2020-12-09 修回日期:2021-03-17 出版日期:2021-07-25 发布日期:2021-07-29
  • 通讯作者: 徐增莱
  • 作者简介:徐明,男,1993年出生,甘肃镇原人,硕士,研究方向:植物资源学。通信地址:210014 江苏南京玄武区前湖后村1号 江苏省中国科学院植物研究所药用植物研究中心,Tel:18061770669,E-mail: 1249568430@qq.com

5 Polygonum tinctorium Ait. Populations: Genetic Diversity Analysis Based on ISSR Marker

Xu Ming(), Wang Qiong, Li Dongling, Zheng Yuhong, Xu Zenglai()   

  1. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014
  • Received:2020-12-09 Revised:2021-03-17 Online:2021-07-25 Published:2021-07-29
  • Contact: Xu Zenglai

摘要:

旨在分析5个不同居群蓼蓝的遗传多样性及亲缘关系,为蓼蓝种质资源的保护和开发利用提供分子依据。以5个不同居群的蓼蓝为实验材料,利用简单重复序列(ISSR)分子标记技术进行遗传多样性分析;使用Popgene 32软件对扩增结果进行分析;使用NTsys2.10e软件按非加权配对算数平均法(UPGMA)构建居群间遗传距离聚类图。结果表明,23条引物共扩增出155个位点,其中148个为多态性位点;Nei′s基因多样性指数(H)为0.3538,Shannon多样性指数(I)为0.5234,基因分化系数(Gst)为0.4096,基因流(Nm)为0.7206;以遗传距离0.234为阈值可将5个居群分为三类,其中日本居群和其他居群间显示出较大差异。本研究结果显示蓼蓝居群间遗传差异与地理距离呈现一定相关性,对蓼蓝遗传多样性及种质资源的保护利用提供了理论基础。

关键词: 蓼蓝, ISSR分子标记, 遗传多样性, 聚类分析

Abstract:

The research aims at analyzing genetic diversity and relationship of 5 Polygonum tinctorium Ait. populations, and providing molecular references to the germplasm resources protection, exploitation and utilization of P. tinctorium Ait.. 5 P. tinctorium Ait. populations were used as materials. The genetic diversity was studied by simple inter-sequence repeat (ISSR) makers. The software Popgene 32 was used to analyze the amplification results, and the NTsys2.10e was used to construct a cluster map according to the unweighted pair-group method with arithmetic means (UPGMA). The results showed that a total of 155 loci were amplified by 23 primers, of which 148 were polymorphic loci; the Nei's genetic diversity coefficient (H) was 0.3538, Shannon's Information index (I) was 0.5234, the coefficient of genetic differentiation (Gst) was 0.4096, and the gene flow (Nm) was 0.7206. Taking the genetic distance 0.234 as a threshold, the 5 populations were divided into three groups, among which the Japanese population was different from others. The results indicate that the genetic difference among the populations of P. tinctorium Ait. is related to the geographical distance. This study could provide a theoretical basis for the genetic diversity of P. tinctorium Ait. and the protection and utilization of germplasm resources.

Key words: Polygonum tinctorium Ait., ISSR molecular marker, genetic diversity, cluster analysis

中图分类号: