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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 104-109.

• 林学 园艺 园林 • 上一篇    下一篇

建兰花香成分的GC-MS分析

杨慧君 姚娜 李潞滨 杨柳 闫伟   

  • 收稿日期:2010-12-31 修回日期:2011-03-02 出版日期:2011-07-05 发布日期:2011-07-05
  • 基金资助:

    海南省重大科技研发专项

GC-MS Analysis of Cymbidium ensifolium Volatile Components

  • Received:2010-12-31 Revised:2011-03-02 Online:2011-07-05 Published:2011-07-05

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摘要：

研究建兰花香成分，为中国兰花花香成分的分析提供依据。以建兰品种‘小桃红’为试材，采用SPME/GC-MS联用技术分析‘小桃红’花香成分。通过正交试验设计，运用极差分析，结果得到‘小桃红’样品前处理优化条件是鲜重为0.6 g，萃取时间为60 min，手柄刻度为4 cm，解吸温度为250℃；将正交试验样品前处理优化条件运用于花香成分分析中，共鉴定出挥发性成分35种，占总百分含量的99.499%，其中，酯类物质含量最多，达到83.155%，是建兰品种‘小桃红’的主要香气成分，其次为醇类，占10.710%；烷类占1.944%；萜烯类占1.713%；酸类占1.659%；酮类占0.319%。结果表明建兰主要花香成分为茉莉酸甲酯（21.563%）、茉莉酮酸甲酯（19.628%）和金合欢醇（10.710%）。

关键词: 方差分析, 方差分析

Abstract:

For providing the bases and establishing the foundation for the analysis of the aroma components of Chinese orchid to research the aroma components of Cymbidium ensifolium. The aroma components of Cymbidium ensifolium ‘Xiao Tao Hong’ were detected by SPME/GC-MS. The suitable condition was gained that was optimized pretreatment of the sample by orthogonal design of experiment and the analysis of range. The optimized condition of pretreatment sample involved weight of fresh flower was 0.6 g, extraction time was 60 min, handle scale was 4 cm and desorption temperature was 250℃.The condition was used in the research for obtaining the credible statistics. There were 35 kinds of components was identified in total and the relative contents was 99.499%. The ester material was the most, about 83.155%, and it was believed that the ester material was the main matter of Cymbidium ensifolium ‘Xiao Tao Hong’. The others were alcohols 10.710%, silane 1.944%, terpenes 1.713%, acids 1.659% and keton 0.319%. The consequences indicated that the major components were Methyl jasmonate (21.563%); Methyl epijasmonate (19.628%) and farnesol (10.710%).