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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (23): 29-31.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

透明带诱导绵羊精子顶体反应的研究

刘烈琴 顾申 周卫宾 杨飞鸽 张宏 郭勇 刘云海 倪和民   

  • 收稿日期:2011-01-04 修回日期:2011-03-20 出版日期:2011-09-15 发布日期:2011-09-15
  • 基金资助:

    国家自然科学基金“MUC1协调GLYCAM1和ISG15调控绵羊子宫容受态机制的研究”

The study of ovine sperm acrosome reaction induced by zone pellucide

  • Received:2011-01-04 Revised:2011-03-20 Online:2011-09-15 Published:2011-09-15

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摘要：

为探讨绵羊透明带（zona pellucida，ZP）对其精子顶体反应的诱导作用，实验分为3组：（1）不经获能处理的精子与透明带共孵育；（2）精子经获能处理后与透明带共孵育；（3）不经获能处理的精子与透明带共孵育，然后IA23187（添钙离子载体，calcium ionophore A23187），来观察透明带对精子顶体反应的诱导效果。结果显示获能与否并不影响透明带对精子顶体反应的诱导效果，添加IA23187后，顶体反应(acrosome reaction，AR)升高，但差异不显著（P>0.05）。说明透明带可诱导绵羊精子(无论获能与否)发生顶体反应，IA23187不能提高透明带对顶体反应的诱导作用。

关键词: 研究应用, 研究应用

Abstract:

To find out the effects of ovine sperm acrosome reaction induced by zone pellucid, three experiments were designed: first, co-incubation non-capacited sperm with zone pellucid; second, co-incubation capacitation sperm with zone pellucid; third, adding with calcium ionophore A23187 after co-incubation non-capacited sperm with zone pellucid. The results showed that the rates of acrosome reaction induced by zone pellucid are not significant difference whether the spermatozoa were capacited, IA23187 don’t cooperate with zone pellucide to improve the rate of acrosome reaction.

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