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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (23): 32-35.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

补硒对萨福克羊血清激素水平和超排效果的影响

吴森 陈福财 王燕燕 周占琴   

  • 收稿日期:2011-04-06 修回日期:2011-05-08 出版日期:2011-09-15 发布日期:2011-09-15
  • 基金资助:

    国家现代肉羊产业技术体系

Effects of selenium supplement on the serum hormone concentration and superovulation effect of Suffolk sheep

  • Received:2011-04-06 Revised:2011-05-08 Online:2011-09-15 Published:2011-09-15

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摘要：

[目的]为了探讨补硒对萨福克羊血清激素水平和超排效果的影响， [方法]将舍饲条件下的24头2岁萨福克母羊随机分为试验组和对照组，试验组在超排处理前肌肉注射亚硒酸钠VE注射液4ml/只(含硒0.46mg/ml)，对照组未注射；到第25天进行胚胎移植时，检查排卵情况，同时采集血样，测定血清P4、E2、FSH、LH、FT3和FT4水平。结果表明：试验组平均获有效胚胎8.7枚，比对照组高2.1枚。试验组血清P4和E2分别为8.46 ±4.54ng/ml和13.49±4.41pg/ml，显著高于对照组(P< 0.05)。血清FSH和LH分别为5.46±1.55和4.12±1.19mIU/ml，虽然高于对照组，但差异不明显。血清FT3、FT4分别为4.67±0.71和10.23±2.32 pmol/L，其中FT4显著低于对照组（P< 0.05）。 [结论]由此可见，注射亚硒酸钠VE注射液可以提高萨福克羊的排卵数和血清P4和E2水平。

关键词: 生物防治, 生物防治

Abstract:

In order to ascertain the effects of selenium supplement on the serum hormone concentration and superovulation effect of donor sheep, 24 two-years old Suffolk managed under stall-feeding condition were divided into treatment and control group randomly. The treatment group was injected with sodium selenite vitamin E injection 4 ml (i.e.0.46 mg/ml) before superovulation treatment, and control group was injected with nothing. The quality and quantity of embryo of Suffolk sheep were evaluated along with the embryo transfer, the serums were prepared to determine the levels of P4、E2、FSH、LH、FT3 and FT4 at 25th-day after treatment. The results showed that 8.7available embryos were collected from treatment group, that was 2.1 more than that of control group. The serum P4、E2 concentrations of treatment group were 8.46 ±4.54ng/ml and 13.49±4.41pg ml respectively，both of them were significantly more than that of control group(P< 0.05). The serum FSH and LH levels of treatment group were 5.46±1.55and 4.12±1.19mIU/ml, there was no obvious difference between two groups. The serum FT3 and FT4 levels were 4.67±0.71and 10.23±2.32 pmol/L respectively，FT4 level was significantly lower than that of control group（P< 0.05. Hence, the quantity of available embryo and serum P4 and E2 of Suffolk sheep used for donor were improved by injection with Sodium selenite vitamin E injection.