欢迎访问《中国农学通报》,

中国农学通报 ›› 2011, Vol. 27 ›› Issue (9): 206-211.

• 农学 农业基础科学 • 上一篇    下一篇

小花南芥根(Arabis alpinal var. parviflora Franch)对铅锌吸收的药理学研究

王吉秀 太光聪 祖艳群 李元 陈海燕   

  • 收稿日期:2011-01-07 修回日期:2011-03-16 出版日期:2011-04-25 发布日期:2011-04-25
  • 基金资助:

    教育厅基金项目;国家自然科学基金

A Pharmacology Study on the Absorption of Lead and Zinc in Roots of Arabis alpinal var. parviflora Franch

  • Received:2011-01-07 Revised:2011-03-16 Online:2011-04-25 Published:2011-04-25

摘要:

以药理学的方法进行水培试验,研究小花南芥根对铅锌的吸收机理。结果表明:小花南芥在50 μmol/L的解偶联剂DNP处理12 h、24 h和36 h后与对照相比根中铅的浓度分别增加了4.4倍,7.1倍和1.7倍,ATP酶抑制剂Na3VO4作用下,则下降了0.64倍,0.76倍和0.69倍;而小花南芥在50 μmol/L的解偶联剂DNP处理24 h和36 h后与对照相比根中锌的浓度分别下降了8.8%和5.4%,ATP酶抑制剂Na3VO4处理对小花南芥根吸收锌不产生影响。钾离子通道抑制剂TEA处理,小花南芥根吸收铅受到明显的抑制作用,不同处理与对照相比下降范围在0.51~0.82倍之间,钙离子通道抑制剂LaCl3处理,下降范围在0.50~0.97倍之间,而小花南芥根吸收锌在TEA处理12 h和24 h后与对照相比下降了0.94倍和0.58倍,LaCl3处理24 h和36 h后与对照相比下降了6.6%倍和9.4%。从上述分析知,能量代谢和离子通道抑制剂对于小花南芥根部铅的吸收产生的影响大于锌的吸收。

关键词: 药剂筛选, 药剂筛选

Abstract:

The water culture experiment is to study the mechanism of Arabis alpinal Var. parviflora Franch’s absorption in Lead and Zinc with a pharmacological approach. The findings show that after the 50μmol.L-1uncoupler DNP treatment 12 h, 24 h and 36 h, the concentration of lead of Arabis alpinal Var. parviflora Franch’s roots increases by 4.4 times, 7.1 times and 1.7 times compared with the control ones. Under the action of ATP inhibitors Na3VO4, the concentration decreases 0.64 times, 0.76 times and 0.69 times. However, after the 50 μmol/L uncoupler DNP treatment 12 h, 24 h and 36 h, the concentration of zinc of Arabis alpinal Var. parviflora Franch’s roots decreases by 8.8 times and 5.4 times. The action of ATP inhibitors Na3VO4 has no impact on the zinc absorption of Arabis alpinal Var. parviflora Franch. Under the potassium channel inhibitor TEA treatment, Arabis alpinal Var. parviflora Franch’s root absorption of lead was significantly restrained. The decreased range is between 0.51 and 0.82 times compared with the control ones with different treatments. After the Calcium channel inhibitor LaCl3 treatment, a decrease is in the range between 0.50 to 0.97 times. While Arabis alpinal Var. parviflora Franch’s root absorption of zinc decreases by 0.94 and 0.58 times in comparison with the controlled after the TEA treatment and by 6.6% times and 9.4% times after the LaCl3 treatment 24 h and 36 h. It can be concluded from the above analysis that energy metabolism and ion channels have a greater impact on Arabis alpinal Var. parviflora Franch’s root lead absorption than zinc absorption.