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中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 57-60.

所属专题: 生物技术 畜牧兽医 园艺

• 林学 园艺 园林 • 上一篇    下一篇

发酵油茶枯饼生产蛋白饲料的混合菌种的筛选

马力 陈永忠 彭邵锋 陈隆升 王湘南 王瑞 唐炜   

  • 收稿日期:2011-01-12 修回日期:2011-03-10 出版日期:2011-07-05 发布日期:2011-07-05
  • 基金资助:

    国家林业局公益类行业专项油茶“两系”杂交种子园营建及其高产培育技术研究

Screening of the Multi-strains to Produce Cell Protein Feeds from Oil-tea Camellia Cake

  • Received:2011-01-12 Revised:2011-03-10 Online:2011-07-05 Published:2011-07-05

摘要:

以油茶枯饼为原料,筛选生产优质高蛋白饲料的菌种为目的。通过平板点种试验,选用黑曲霉为指示菌,又根据刺激圈试验以及三角瓶发酵培养,获得油茶枯饼生产蛋白饲料的最佳菌种组合,油茶枯饼发酵后粗蛋白含量为16.94%,较未发酵时的10.82%提高了56.56%。试验结果表明,油茶枯饼生产蛋白饲料的最佳菌种组合为:地衣芽孢杆菌10181︰黑曲霉︰葡萄汁酵母1445︰米曲霉=1︰1︰1︰1时为优选接种比例。

关键词: 甘蓝 芜菁花叶病毒(TuMV)抗性, 甘蓝 芜菁花叶病毒(TuMV)抗性

Abstract:

The research aim to select strains producing high quality protein feedstuff in oil-tea Camellia cake as raw material. The strain of Aspergilluse niger was selected to be an indicator strain through the dot-in-oculating method. The optimum multi-strains ratio of fermenting multi-strains was selected according to the results from the stimulating circle experiment as well as fermentation experiment in the flask. The protein content of fermen-ring product under this fermentation condition could reach 16.94%, which was 56.56% higher than the control of 10.82%. Then the optimum multi-strains ratio of fermenting multi-strains was Bacillus licheniformis10181︰Aspergilluse niger︰Saccharomyces uvarum1445︰Aspergilluse niger=1︰1︰1︰1.