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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 61-64.

所属专题： 资源与环境

• 林学 园艺 园林 • 上一篇    下一篇

利用RAMP-PCR技术对枸杞10个品种资源的分析

梁海永 刘兴菊 杨敏生   

  • 收稿日期:2011-01-26 修回日期:2011-03-02 出版日期:2011-07-05 发布日期:2011-07-05
  • 基金资助:

    国家林业局行业公益项目“北方主要林木品种指纹库构建及分子鉴定技术研究”

Analysis of Lycium chinense Variety Revealed by RAMP-PCR Method

  • Received:2011-01-26 Revised:2011-03-02 Online:2011-07-05 Published:2011-07-05

PDF (PC)

898

摘要：

以枸杞为材料，建立基因组DNA的有效提取方法，并对10个枸杞品种进行了RAMP技术分析研究。结果表明，利用10对引物共扩增出87个条带，其中多态性条带53个；多态性条带比率33.3%~81.9%，10个枸杞品种间的遗传距离为0.1111~0.8333；研究表明，利用RAMP进行枸杞的品种DNA指纹分析是可行的。

关键词: 种植业结构, 种植业结构, 现状, 评价, 聊城市

Abstract:

To develop valid methods of DNA extraction for Chinese wolfberry for RAMP analysis and research. The result showed that: ten primers were applied to amplification. A total of 87 DNA bands were detected. 53 bands among which were polymorphic. The rate of polymorphic bands was 33.3%-81.9%. 10 Chinese wolfberry cultivars genetic distances were from 0.1111 to 0.8333. In the study, the RAMP markers can be used for the studies of fingerprint of Chinese wolfberry sensitively.