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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (23): 114-118.

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

燕窝蛋白质样品制备方法的比较及其双向电泳分析

黄秀丽 赖心田 张世伟 林霖 詹家芬 林俊芳 杨国武   

  • 收稿日期:2011-01-24 修回日期:2011-02-24 出版日期:2011-09-15 发布日期:2011-09-15

Comparison of Protein Preparation and Two Dimensional Electrophoresis Analysis from Edible Bird’s Nest

  • Received:2011-01-24 Revised:2011-02-24 Online:2011-09-15 Published:2011-09-15

PDF (PC)

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摘要：

燕窝水提及丙酮沉淀蛋白质经液相等电聚焦电泳纯化后，再经双向电泳分离获得了质量较佳的2-DE图谱，由此可见液相等电聚焦电泳是对燕窝蛋白质进行纯化的有效手段。对纯化后的2个白燕窝和1个血燕窝水提及丙酮沉淀蛋白质进行了双向电泳分析，结果显示两种方法制备蛋白质获得的2-DE图谱非常相似。燕窝水提蛋白质经丙酮沉淀制备样品时间较透析、冻干所需的时间短，这对于成分更为复杂且蛋白质提取率较低的血燕窝则更为适用。

关键词: 免疫学特性, 免疫学特性

Abstract:

Protein samples, prepared with water extraction or acetone precipitation, were both well separated by two-dimensional electrophoresis (2-DE) after purification with liquid-phase isoelectric focusing (LIEF). So LIEF was an effective purification method for edible birds’ nest (EBNs). With different preparation methods, similar 2-D gel protein profiles were obtained in two white EBNs and one blood EBN. While compared to dialyzation and lyophilization, the time of sample preparation with acetone precipitation was greatly reduced. Consequently, protein precipitated with acetone prior to LIEF was more applicable to blood EBNs for their low protein extraction rate.