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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (23): 119-122.

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

豆粉基因组DNA不同提取方法的比较

徐伟丽 马莺 杜明 汪家琦 李启明   

  • 收稿日期:2011-01-13 修回日期:2011-03-03 出版日期:2011-09-15 发布日期:2011-09-15
  • 基金资助:

    四川省应用基础研究;哈尔滨工业大学优秀青年教师培养计划

Comparison of Different Extraction Methods of Genomic DNA from Soybean Powder

  • Received:2011-01-13 Revised:2011-03-03 Online:2011-09-15 Published:2011-09-15

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摘要：

以市售豆粉为材料，分别采用热解法、异丙醇沉淀法、CTAB法、SDS法、高盐低pH法等以及它们的改良方法提取基因组DNA，并对提取的DNA进行光密度、琼脂糖凝胶电泳和PCR检测。结果表明除改进高盐低pH法外，其它所有方法提取的基因组DNA均可满足PCR检测要求。同时，综合考虑基因组DNA的纯度和浓度，此研究认为豆粉基因组DNA提取方法的优劣依次为：改进热解法、改进异丙醇沉淀法、热解法、异丙醇沉淀法、高盐低pH法、改进CTAB法、CTAB法和改进SDS法。这几种豆粉基因组DNA提取方法都具有操作简单、耗时短、利于快速检测的优点。

关键词: 降血脂, 降血脂

Abstract:

In the present study, to find out the better methods of soybean powder genomic DNA extraction for the molecular marker analysis of high quality and sufficient samples, the extraction method of genomic DNA from soybean powder purchased from market was optimized. There are 12 methods used in the extraction of DNA, in which pyrolysis, high-salt low-pH, isopropyl alcohol, CTAB, SDS or GuSCN, and their modified methods were involved. The effects of different methods were compared by detecting the DNA by optical density, agarosegel electrophoresis and polymerase chain reaction (PCR). Results showed that the genomic DNA extracted by all methods except for modified High-salt low-pH method could be used in PCR reaction. Meanwhile, the better DNA concentration and purity will be gained by different methods in the order of modified pyrolysis method, modified isopropyl alcohol method, pyrolysis method, isopropyl alcohol method, High-salt low-pH method, modified CTAB method, CTAB method and modified SDS method. These methods are simply to operate, fast to gain results, and suitable for the extraction of total DNA from soybean powder.