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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (23): 123-127.

• 食品 营养 检测 安全 • 上一篇    下一篇

有机溶剂盐溶液分离二醇型人参皂苷的研究

邓小莉 常景玲 张卓 吴羽晨   

  • 收稿日期:2011-03-08 修回日期:2011-04-13 出版日期:2011-09-15 发布日期:2011-09-15
  • 基金资助:

    河南省重点科技攻关项目“人参皂苷Rb1微生物转化研究”

Study of Separating Glycol Type Ginseng Saponins from Organic Solvents CaCl2 Salt Solution

  • Received:2011-03-08 Revised:2011-04-13 Online:2011-09-15 Published:2011-09-15

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摘要：

为确定分离二醇型人参皂苷最佳的有机溶剂及其用量。采用二醇型人参皂苷单体在有机溶剂CaCl2盐溶液中的不同溶解度进行萃取，经高效液相色谱检测，得出最优的有机溶剂及其用量。结果显示丙酮CaCl2盐溶液分离二醇型人参皂苷效果最佳。分离萃取二醇型人参皂苷最佳条件为皂苷(g):5% CaCl2(mL):丙酮(mL)=4:5:35。

关键词: 派酶-土壤-根系复合体, 派酶-土壤-根系复合体, 黑麦草, 抗剪强度

Abstract:

This study is to determine the best organic solvents and the dose of all monomers in separating glycol type ginseng saponins. Through extraction by using the different solubilities of the monomers of glycol type ginseng saponins in organic solvents CaCl2 salt solution, the study determines the best Organic solvents and its dose by using the High Performance Liquid Chromatography test. The test comes to the conclusion that Acetone CaCl2 salt solution is the best organic solvents in separating glycol type ginseng saponins and the best condition for the extraction of glycol type ginseng saponins is saponins(g):5%CaCl2(mL):Acetone(mL)=4:5:35.

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