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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (17): 83-86.

• 食品 营养 检测 安全 • 上一篇    下一篇

桔梗多糖的分离纯化与含量测定

贾林 陆金健 卢德赵 施佳杰 周文雅 沃兴德   

  • 收稿日期:2011-03-08 修回日期:2011-04-25 出版日期:2011-07-15 发布日期:2011-07-15

Purification and Content Determination of the Polysaccharides from Platycodon grandiflorum

  • Received:2011-03-08 Revised:2011-04-25 Online:2011-07-15 Published:2011-07-15

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摘要：

利用离子交换凝胶柱层析法分离纯化桔梗多糖，并对纯化组分进行纯度鉴定和糖含量及蛋白质含量测定。桔梗饮片经热水浸提、乙醇分步沉淀得桔梗粗多糖PPS80，Sevage法脱蛋白后，通过DEAE-Sepharose FF离子交换和Sephadex G-75凝胶柱层析进行纯化鉴定。分别采用苯酚-硫酸法和考马斯亮蓝染色法测定纯化后各组分的糖和蛋白质含量。试验结果表明，从桔梗粗多糖PPS80中分离得到2个均一性组分PPS80-I和PPS80-II，糖含量分别为90.32%和86.75%，蛋白质含量分别为0.48%和1.96%。该试验首次采用DEAE-Sepharose FF离子交换柱层析结合Sephadex G-75凝胶柱层析有效分离纯化出桔梗多糖均一性组分，为桔梗多糖的进一步研究提供基础。

关键词: 碧玉兰, 碧玉兰, 多倍体诱导, 秋水仙素

Abstract:

To purify the polysaccharide from Platycodon grandiflorum via ion-exchange chromatography and detect the contents of saccharide and protein. The crude polysaccharide named PPS80 was extracted from Platycodon grandiflorum using hot water-ethanol fractional precipitation. After removing the protein by Sevage method, the polysaccharide was purified and identified with DEAE-Sepharose FF ion-exchange chromatography and Sephadex G-75 gel chromatography, respectively. The contents of saccharide and protein were determined by phenol-sulfate acid method and Coomassie brilliant blue staining assay, respectively. The results showed that two homogeneous constituents were isolated from PPS80 named as PPS80-I and PPS80-II. It was determined that PPS80-I contained 90.32% saccharides and 0.48% proteins while PPS80-II contained 86.75% saccharides and 1.96% proteins, respectively. Two homogeneous constituents of polysaccharides were efficiently purified from the crude polysaccharide PPS80 via DEAE-Sepharose FF ion-exchange chromatography and Sephadex G-75 gel chromatography. These results laid an experimental foundation for further research on the polysaccharides from Platycodon grandiflorum.