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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (27): 1-5.doi: 10.11924/j.issn.1000-6850.casb2021-0999

所属专题： 生物技术

• 农学·农业基础科学 •    下一篇

秋水仙素诱导甜高粱‘FH59’种子变异效应研究

邵荣峰(), 张伟, 张阳, 王官, 常玉卉, 白文斌, 赵威军()   

  1. 山西农业大学高粱研究所,山西晋中 030600
  • 收稿日期:2021-10-20 修回日期:2022-03-20 出版日期:2022-10-05 发布日期:2022-09-21
  • 通讯作者: 赵威军
  • 作者简介:邵荣峰,男,1985年出生,山西昔阳人,助理研究员,本科,主要从事甜高粱遗传育种研究。通信地址：030600 山西省晋中市榆次区蕴华西街238号 山西农业大学高粱研究所,Tel：15035677501,E-mail： syf3362@126.com。
  • 基金资助:
    山西农业大学生物育种工程“抗蚜甜高粱亲本系创制及新品种选育”(YZGC060);高粱遗传与种质创新山西省重点实验室青年基金项目“秋水仙素诱导甜高粱染色体加倍效应研究”(2018Q-4)

Mutagenic Effect of Colchicine on Sweet Sorghum ‘FH59’ Seeds

SHAO Rongfeng(), ZHANG Wei, ZHANG Yang, WANG Guan, CHANG Yuhui, BAI Wenbin, ZHAO Weijun()   

  1. Sorghum Institute, Shanxi Agricultural University, Jinzhong, Shanxi 030600
  • Received:2021-10-20 Revised:2022-03-20 Online:2022-10-05 Published:2022-09-21
  • Contact: ZHAO Weijun

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摘要：

为了研究秋水仙素水溶液对甜高粱‘FH59’种子的诱变效应,创制优质甜高粱种质资源。本实验利用不同浓度的秋水仙素水溶液(0.05%、0.1%、0.15%、0.2%)浸泡甜高粱种子24、31、48 h,统计苗期变异率,并对成活植株进行大田鉴定。研究结果表明,秋水仙素诱变效果受到处理时间、药剂浓度以及二者交互作用的显著影响,在24 h,0.15%诱变条件下诱变效果最佳,幼苗变异效果显著。M2代田间鉴定发现株高、生育期、穗长、籽粒颜色、锤度等主要农艺性状的突变体,变异类型丰富,尤其是发现的大量的矮化、早熟突变体对甜高粱亲本的改良作用明显。

关键词: 秋水仙素, 甜高粱, 诱变效应, 矮化, 种质资源

Abstract:

In order to study the mutagenic effect of colchicine aqueous solution on sweet sorghum ‘FH59’ seeds and create high-quality sweet sorghum resources, in this experiment, sweet sorghum seeds were soaked in different concentrations of colchicine solution (0.05%, 0.1%, 0.15%, and 0.2%) for 24 h, 31 h and 48 h, respectively, the variation rate at seedling stage was counted, and the survival plants were identified in field. The results showed that the mutagenic effect of colchicine was significantly affected by the treatment time and the concentration of colchicine and their interaction. The best mutagenic effect was obtained under the condition of 0.15% colchicine solution treatment for 24 h, and the mutagenic effect of seedlings was significant. The mutants of plant height, growth period, ear length, grain color, brix and other major agronomic traits were found in the field of M2 generation, and the variation types were rich. Especially, a large number of dwarfing and early-maturing mutants were found, which had obvious improvement on sweet sorghum parents.

Key words: colchicine, sweet sorghum, mutagenic effect, dwarfing, germplasm resource

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