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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (20): 97-102.

所属专题： 水产渔业

• 水产 渔业 • 上一篇    下一篇

亚硝酸盐胁迫下丁酸梭状芽孢杆菌对日本沼虾应激代偿的调节

吴伟   

  • 收稿日期:2011-03-25 修回日期:2011-04-09 出版日期:2011-08-15 发布日期:2011-08-15
  • 基金资助:

    无锡市院士工作站项目

The Effects of Clostridium butyricum in Superoxide Dismutase (SOD) and Catalase (CAT) Activity Response to Nitrite Stress in Macrobrachium nipponense

  • Received:2011-03-25 Revised:2011-04-09 Online:2011-08-15 Published:2011-08-15

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摘要：

以日本沼虾（Macrobrachium nipponense）为试验材料，研究了在亚硝酸盐的污染胁迫下，饲料中添加一定量的丁酸梭状芽孢杆菌（Clostridium butyricum）对试验沼虾的生长及肌肉组织中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性的影响。研究结果表明，经21d的试验，添加1%和5%的丁酸梭状芽孢杆菌可显著提高沼虾的生长率、SOD和CAT的活性（P<0.01），但2个添加组之间的差异不明显（P>0.05）。当养殖水环境中的亚硝酸钠浓度在0~0.50 mg?L-1范围内，试验组沼虾肌肉的SOD和CAT活性随着亚硝酸钠质量浓度和丁酸梭状芽孢杆菌添加量的上升而升高，在试验的第6~9d时达到峰值，随后又开始下降。至试验21d时，沼虾肌肉组织中SOD和CAT活性取决于水环境中亚硝酸钠的质量浓度和饲料中丁酸梭状芽孢杆菌的添加量。研究表明饲料中添加丁酸梭状芽孢杆菌不仅可促进日本沼虾的生长，而且还可以增加日本沼虾对亚硝酸盐污染胁迫的耐受和抵抗能力，其适宜的添加量为106个活菌数/克饲料。

关键词: 延长随机引物扩增DNA, 延长随机引物扩增DNA

Abstract:

The new breed “Taihu Lake No.1" (Macrobrachium nipponense) was fed with basal diet supplemented with different concentration treatments of Clostridium butyricum under nitrite stress. The study was conducted to explore the effects of C. butyricum on isoenzymes of superoxide dismutase (SOD) and catalase (CAT) in muscles of M. nipponense. Results showed that the growth rate and the activity of SOD and CAT were significantly improved in the 1％ and 5％ treatment after 21days (P<0.01), and there was no significant different between these two groups (P>0.05).When the mass concentration of nitrite in water environment was in 0~0.50 mg?L-1, the activity of SOD and CAT in muscle was enhanced with the increase in the concentration of nitrite and C. butyricum, which reached to the top in 6 to 9day and then decreased. In 21 day, the activity of SOD and CAT in muscle depends on the concentration of nitrite-Na in water environment and supplement of different C. butyricum level on feed. All results indicated that supplement of C. butyricum on feed improved growth and alleviated the damage of nitrite stress. The optimal supplementation of C. butyricumw was 106 cfu/g.

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