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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (28): 139-144.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

山苦茶组织培养与快速繁殖研究

梁柳 王和飞 刘进平   

  • 收稿日期:2011-05-06 修回日期:2011-06-17 出版日期:2011-11-05 发布日期:2011-11-05
  • 基金资助:

    山苦茶组织培养及扦插繁殖技术研究

The Study on Culture and Rapid Propagation of Mallotus furetianus

  • Received:2011-05-06 Revised:2011-06-17 Online:2011-11-05 Published:2011-11-05

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摘要：

为了给山苦茶（Mallotus oblongifolius）大规模繁殖和推广种植打下技术基础，以山苦茶当年生带芽茎段为试验材料，研究了山苦茶离体茎段培养和快速繁殖的影响因素。结果表明山苦茶带芽茎段最佳消毒方法为70%的酒精浸泡30 s，0.1% HgCl2溶液消毒8 min。最适宜的山苦茶茎段启动培养基为MS+2.0 mg/L 6-BA+1.5 mg/L KT。1/2MS为基本培养基，细胞分裂6-BA和KT组合有利于嫩茎增殖。山苦茶较好的继代增殖培养基为1/2MS+2.0 mg/L 6-BA+1.5 mg/L KT，生根培养基为1/2MS+2.0 mg/L IBA+2.0 mg/L NAA。利用本试验所得出的结果，可实现山苦茶种苗的离体无性快速繁殖，增殖率可达3。

关键词: 蛋白纯化, 蛋白纯化, 包涵体, SDS-PAGE, KCl染色

Abstract:

In order to provide base of large scale propagation and plantation of Mallotus furetianus, factors influenced on in vitro culture and rapid propagation of Mallotus furetianus were studied using stem segments with buds as explants. The results showed that the best sterilization method for the stem segments was dipping in 70% ethanol for 30 s, and then agitating in 0.1% HgCl2 for 8 min. The optimum medium for initiation culture of stem segments was MS with 1.5 mg/L KT and 2.0 mg/L 6-BA. The basic medium 1/2MS with the combination of 6-BA and KT could enhance the multiplication. The suitable multiplication medium was 1/2MS+2.0 mg/L 6-BA +1.5 mg/L KT and the suitable rooting medium was 1/2MS+2.0 mg/L IBA+2.0 mg/L NAA. Using the methods obtained in this experiment, in vitro rapaid propagation of Mallotus oblongifolius can be achieved at the multiplication rate of 3.

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