欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (28): 36-39.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

黄檗茎段不定芽分化过程中生理生化指标变化研究

王慧梅 于海娣 陈亚杰   

  • 收稿日期:2011-05-09 修回日期:2011-07-18 出版日期:2011-11-05 发布日期:2011-11-05

Studies on Changes of Physiology and Biochemistry Indexes During Adventitious Shoot Regeneration from Stem Explants of Phellodendron amurense

  • Received:2011-05-09 Revised:2011-07-18 Online:2011-11-05 Published:2011-11-05

PDF (PC)

1010

摘要：

以黄檗茎段为外植体，研究其不定芽分化过程中生理生化指标的变化。将黄檗无菌苗的茎段接种到MS+1.5 mg/L BA+0.5 mg/L NAA+20 g/L葡萄糖的培养基上，于20天左右大量形成不定芽，30天左右形成小苗。通过比色法，对茎段不定芽再生过程的生理生化指标测定表明，在不定芽形成过程中，SOD酶活性呈现出逐渐下降的趋势，而其他2种抗氧化酶（CAT和POD）活性、可溶性蛋白及可溶性糖含量在不定芽形成的高峰期之前均呈不同程度的上升趋势，随后则呈下降趋势。实验表明，抗氧化酶活性，可溶性蛋白及可溶性糖含量与不定芽再生有着明显的相关性。

关键词: Atfad8, Atfad8, 叶绿体转基因烟草, 冷胁迫, 生理响应

Abstract:

The stem segments were used as explants to study the changes of physiology and biochemistry indexes during adventitious shoot regeneration.The stem segments from aseptic seedling of Phellodendron amurense were inoculated on MS medium containing 1.5 mg/L 6-BA, 0.5 mg/L NAA and 20 g/L glucose. After about 20 days of culture adventitious shoots were induced, and occurred to regenerated plantlets at 30 days or so. By using colorimetry, the SOD activity showed downtrend during the formation of adventitious shoots. While the other antioxidant enzyme (CAT and POD) activities, the concent of soluble protein as well as soluble sugar all increased in varying degrees before peak of adventitious shoot formation, and then decreased. The results showed that there was obvious correlation between antioxidant enzyme activities, the concent of soluble protein and soluble sugar and adventitious shoot regeneration.