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中国农学通报 ›› 2017, Vol. 33 ›› Issue (31): 33-37.doi: 10.11924/j.issn.1000-6850.casb16100029

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

外源亚精胺对高温胁迫下萝卜幼苗生长发育的影响

梅燚,祖艳侠,王薇薇,吴永成,郑佳秋,葛兆建,郭军   

  1. 江苏沿海地区农业科学研究所,江苏沿海地区农业科学研究所,江苏沿海地区农业科学研究所,江苏沿海地区农业科学研究所,江苏沿海地区农业科学研究所,江苏沿海地区农业科学研究所,江苏沿海地区农业科学研究所
  • 收稿日期:2016-10-10 修回日期:2016-11-09 接受日期:2016-11-11 出版日期:2017-11-27 发布日期:2017-11-27
  • 通讯作者: 郭军
  • 基金资助:
    江苏省农业科技自主创新项目“萝卜耐热机理研究及相关基因的克隆”[CX(13)5082];盐城市农业科技创新专项“设施叶菜新品种及优质 安全生产技术集成与示范”(YK2014033)

Effects of Exogenous Spermidine on Growth and Development of Radish Seedlings Under High Temperature Stress

  • Received:2016-10-10 Revised:2016-11-09 Accepted:2016-11-11 Online:2017-11-27 Published:2017-11-27

摘要: 以萝卜为试验材料,研究了喷施外源spd对于高温下萝卜幼苗生长的影响。采用营养液栽培萝卜幼苗,在温光培养箱中对植株进行昼夜40°C/30°C的高温胁迫,在不同处理时间喷施1mM的外源亚精胺,并测定了幼苗植株生长量、叶片叶绿素含量、丙二醛、抗氧化酶活性等抗逆相关指标。结果表明:随着高温处理时间的延长,植株生长受到极大的抑制,叶绿素、可溶性蛋白质出现下降,MDA含量升高,过氧化酶活性出现先升高最后降低的规律。喷施外源spd后,能有效促进萝卜幼苗的生长,提高叶绿素和可溶性蛋白质含量,降低MDA的含量,增强过氧化酶的活性,达到清除细胞内产生的活性氧,维持细胞膜结构和细胞内物质的稳定的目的,最终提高萝卜抗高温能力。

关键词: 种植密度, 种植密度, 播期, 农艺性状, 产量

Abstract: The experiment was carried out to study the effects of 1mM exogenous spermidine( Spd) on radish seedling growth, the photosynthetic pigments content in leave, soluble protein content, MDA, Activity of Antioxidant Enzymes. The radish seedling was exposed to high temperature (40℃/30℃) day alternates with night for one to five days with solution culture. The results show that with the increasing duration of high temperature stress seedling growth was significantly inhibited. The amount of photosynthetic pigments content in leaves and soluble protein content of seedlings decreased, and the amount of MDA increased. Antioxidant enzyme activities including POD, CAT, SOD and PAX in seedling increased first, and decreased finally. Exogenous Spd spray could promote the seedling growth. Suitable Spd concentration enhanced the activities of CAT, POD, SOD, APX, photosynthetic pigments, and soluble protein content; while the content of MDA decreased. The Spd concentration can also reduce the production O-2 rate and H2O2 content, maintain the stability and integrity of cell membrane structure. Finally enhanced high temperature tolerance of radish Seedlings.

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