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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (6): 166-172.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

培养基组分对纤维杆菌X4-9发酵产纤维素酶的影响

叶川 李昆太 黄欠如 钟义军 武琳 成艳红   

  • 收稿日期:2011-07-20 修回日期:2011-09-08 出版日期:2012-02-25 发布日期:2012-02-25
  • 基金资助:

    “十一五”国家科技支撑课题“赣东北低丘岗地区红壤退化防治与生态农业技术集成和示范（2009BADC6B04）”

Effects of the Fermentation Medium Components on the Cellulase Production by Cellulomonas X4-9

  • Received:2011-07-20 Revised:2011-09-08 Online:2012-02-25 Published:2012-02-25

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摘要：

考察发酵培养基组分对纤维杆菌X4-9发酵产葡聚糖内切酶、葡聚糖外切酶和β-葡萄糖苷酶的影响。应用DPS软件的二次回归旋转中心组合实验设计方法，对纤维杆菌X4-9的发酵培养基（花生秸杆粉、蔗糖、尿素、(NH4)2SO4、硫酸镁、磷酸二氢钾）进行了优化。结果表明：在试验数值范围内，花生秸杆粉、蔗糖和(NH4)2SO4对酶活均会产生极显著的正效应，而尿素、硫酸镁和磷酸二氢钾的用量则具有明显的负效应；在优化的发酵培养基配方（花生秸杆粉30 g/L、蔗糖20 g/L、尿素2.5 g/L、(NH4)2SO4 10 g/L）下，葡萄糖内切酶、葡聚糖外切酶和β-葡萄糖苷酶的酶活分别达到2.705163，5.723014和3.614921 I U/mL，比初始发酵培养基配方下的各酶活分别提高了123.66%，146.32%和186.40%。纤维杆菌X4-9均能同时产生葡萄糖内切酶、葡聚糖外切酶、β-葡萄糖苷酶等纤维素降解复合酶系，且这些酶高产的培养基条件较为一致。

关键词: 遗传结构, 遗传结构

Abstract:

In this paper, the effects of the components in fermentation medium on the endoglucanase, exoglucanase and β-glucosidase producing by Cellulomonas X4-9 were investigated. The rotational quadratic orthogonal experiment design by DPS soft was used to optimize the fermentation medium (Peanut straw powder, Sucrose, Carbamide, (NH4)2SO4, MgSO4 and KH2PO4) of Cellulomonas X4-9. The results showed that peanut straw powder, sucrose and (NH4)2SO4 had a significantly positive effect on the biosynthesis of the above enzymes, but carbamide, MgSO4 and KH2PO4 caused a negative effect. In the case of the optimal fermentation medium (Peanut straw powder 30 g/L, Sucrose 20 g/L, Carbamide 2.5 g/L, (NH4)2SO4 10 g/L) for Cellulomonas X4-9, the final activity of endoglucanase, eXoglucanase and β-glucosidase was 2.705163, 5.723014 and 3.614921 IU/mL, which was 123.66%, 146.32% and 186.40% higher than that obtained under the original medium recipe, respectively. Cellulomonas X4-9 could produce a group of enzymes including endoglucanase, exoglucanase and β-glucosidase, and the condition of fermentation medium was coincident to the higher productivity.